Yibar Artun, Duman Muhammed, Ay Hilal, Ajmi Nihed, Tasci Gorkem, Gurler Fatma, Guler Sabire, Morick Danny, Saticioglu Izzet Burcin
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, Türkiye.
Department of Aquatic Animal Disease, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, Türkiye.
Pathogens. 2025 Jan 10;14(1):52. doi: 10.3390/pathogens14010052.
Consuming raw or undercooked mussels can lead to gastroenteritis and septicemia due to contamination. This study analyzed the prevalence, density, species diversity, and molecular traits of spp. in 48 fresh raw wild mussels (FRMs) and 48 ready-to-eat stuffed mussels (RTE-SMs) through genome analysis, assessing health risks. The results showed prevalence rates of 12.5% in FRMs and 4.2% in RTE-SMs, with as the most common species (46.7%). It was determined that the seasonal distribution of spp. prevalence in the samples was higher in the summer months. The genome sizes of the spp. ranged from approximately 3.9 to 6.1 Mb, with the GC contents varying between 41.9% and 50.4%. A total of 22 virulence factor (VF) classes and up to six antimicrobial resistance (AMR) genes were detected in different species. The presence of nine different biosynthetic gene clusters (BGCs), 27 prophage regions, and eight CRISPR/Cas systems in 15 strains provides information about their potential pathogenicity, survival strategies, and adaptation to different habitats. Overall, this study provides a comprehensive understanding of the genomic diversity of spp. isolated from FRM and RTE-SM samples, shedding light on the prevalence, pathogenicity, and toxicity mechanisms of -induced gastroenteritis.
食用生的或未煮熟的贻贝可能会因污染导致肠胃炎和败血症。本研究通过基因组分析，对48个新鲜野生生贻贝（FRM）和48个即食酿贻贝（RTE - SM）中的某物种的流行率、密度、物种多样性和分子特征进行了分析，评估健康风险。结果显示，FRM中的流行率为12.5%，RTE - SM中的流行率为4.2%，其中该物种为最常见物种（46.7%）。经测定，样本中该物种流行率的季节分布在夏季月份较高。该物种的基因组大小范围约为3.9至6.1 Mb，GC含量在41.9%至50.4%之间变化。在不同的该物种中总共检测到22类毒力因子（VF）和多达6种抗菌抗性（AMR）基因。在15个该菌株中存在9个不同的生物合成基因簇（BGC）、27个前噬菌体区域和8个CRISPR/Cas系统，这为它们的潜在致病性、生存策略以及对不同栖息地的适应性提供了信息。总体而言，本研究全面了解了从FRM和RTE - SM样本中分离出的该物种的基因组多样性，揭示了该物种引起肠胃炎的流行率、致病性和毒性机制。
