The Austere environments Consortium for Enhanced Sepsis Outcomes (ACESO), The Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, USA.
Naval Medical Research Center, Frederick, Maryland, USA.
BMJ Open. 2021 Sep 15;11(9):e050330. doi: 10.1136/bmjopen-2021-050330.
In 2012, US Marines and Sailors began annual deployments to Australia to participate in joint training exercises with the Australian Defence Force and other partners in the region. During their training, US service members are exposed to a variety of infectious disease threats not normally encountered by American citizens. This paper describes a cohort of US Marines and Sailors enrolled during five rotations to Australia between 2016 and 2020.
Study participation is strictly voluntary. Group informational sessions are held prior to deployment to describe the study structure and goals, as well as the infectious disease threats that participants may encounter while in Australia. All participants provided written informed consent. Consented participants complete a pre-deployment questionnaire to collect data including basic demographic information, military occupational specialty, travel history, family history, basic health status and personal habits such as alcohol consumption. Blood is collected for serum, plasma and peripheral blood mononuclear cells (PBMC) processing. Data and specimen collection is repeated up to three times: before, during and after deployment.
From the five rotations that comprised the 2016-2020 Marine Rotational Force-Darwin, we enrolled 1289 volunteers. Enrolments during this period were overwhelmingly white male under the age of 24 years. Most of the enrollees were junior enlisted and non-commissioned officers, with a smaller number of staff non-commissioned officers and commissioned officers, and minimal warrant officers. Over half of the enrollees had occupational specialty designations for infantry.
In the future, we will screen samples for serological evidence of infection with , , Ross River virus, SARS-CoV-2 and other operationally relevant pathogens endemic in Australia. Antigenic stimulation assays will be performed on PBMCs collected from seropositive individuals to characterise the immune response to these infections in this healthy American population.
2012 年，美国海军陆战队员和水手开始每年部署到澳大利亚，与澳大利亚国防军和该地区的其他伙伴参加联合训练演习。在训练期间，美国军人会接触到各种通常不会被美国公民接触到的传染病威胁。本文描述了 2016 年至 2020 年间在澳大利亚的五次轮调中招募的一组美国海军陆战队员和水手。
研究参与是严格自愿的。在部署前会举行小组信息会议，介绍研究结构和目标，以及参与者在澳大利亚可能遇到的传染病威胁。所有参与者都提供了书面知情同意书。同意的参与者完成一份部署前问卷，收集包括基本人口统计学信息、军事职业专业、旅行史、家族史、基本健康状况和个人习惯（如饮酒）在内的数据。采集血液进行血清、血浆和外周血单核细胞（PBMC）处理。数据和标本采集最多重复三次：部署前、部署期间和部署后。
在 2016-2020 年达尔文海军陆战队轮换期间，我们招募了 1289 名志愿者。在此期间，招募的志愿者绝大多数是年龄在 24 岁以下的白人男性。大多数入伍者是初级 enlisted 和非委任军官，还有较少的参谋 enlisted 和委任军官，以及最少的准尉。超过一半的入伍者有步兵职业专业指定。
在未来，我们将筛选样本，以检测血清中感染 、 、罗斯河病毒、SARS-CoV-2 和其他在澳大利亚流行的与作业相关病原体的证据。我们将对从血清阳性个体中采集的 PBMC 进行抗原刺激检测，以描绘这种健康美国人群对这些感染的免疫反应。
