Emechebe Uchenna, Giraud David, Ammi Azzdine Y, Scott Kristin L, Jacobs Jon M, McDermott Jason E, Dykan Igor V, Alkayed Nabil J, Barnes Anthony P, Kaul Sanjiv, Davis Catherine M
Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, 97239, USA.
Department of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, OR, 97239, USA.
Data Brief. 2021 Sep 4;38:107343. doi: 10.1016/j.dib.2021.107343. eCollection 2021 Oct.
Cardiac endothelial cells respond to both ischemia and therapeutic ultrasound; the proteomic changes underlying these responses are unknown. This data article provides raw and processed data resulting from our global, unbiased phosphoproteomics investigation conducted on primary mouse cardiac endothelial cells exposed to ischemia (2-hour oxygen glucose deprivation) and ultrasound (250 kHz, 1.2 MPa) in vitro [1]. Proteins were extracted from cell lysates and enriched phosphopeptides were analyzed with a high mass accuracy liquid chromatrography (LC) - tandem mass spectrometry (MS/MS) proteomic platform, yielding multiple alterations in both total protein levels and phosphorylation events in response to ischemic injury and ultrasound. This dataset can be used as a reference for future studies on the cardiac endothelial response to ischemia and the mechanistic underpinnings of the cellular response to ultrasound, with the potential to yield clinically relevant therapeutic targets.
心脏内皮细胞对缺血和治疗性超声均有反应；这些反应背后的蛋白质组学变化尚不清楚。本文提供了原始数据和处理后的数据，这些数据来自我们对体外暴露于缺血（2小时氧葡萄糖剥夺）和超声（250kHz，1.2MPa）的原代小鼠心脏内皮细胞进行的全面、无偏倚的磷酸化蛋白质组学研究[1]。从细胞裂解物中提取蛋白质，并用高精度液相色谱（LC）-串联质谱（MS/MS）蛋白质组学平台分析富集的磷酸肽，结果显示，缺血损伤和超声作用下，总蛋白水平和磷酸化事件均发生了多种变化。该数据集可作为未来研究心脏内皮细胞对缺血反应以及细胞对超声反应机制基础的参考，有望产生临床相关的治疗靶点。
