Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.
Mikrochim Acta. 2021 Sep 16;188(10):344. doi: 10.1007/s00604-021-04988-7.
A dual signal-amplified sandwich electrochemiluminescence (ECL) immunosensor was fabricated for trace detection of procalcitonin (PCT). CeO-Au@Pt composed of sea urchin-like Au@Pt nanoparticles coated on CeO hollow nanospheres was immobilized on electrode surface to electrochemically catalyze HO to produce a large number of superoxide anion (O). The immunosensor was prepared by linking the capture antibody on immobilized CeO-Au@Pt with heptapeptide (HWRGWVC), which could maintain the activity of the antibody. The prepared Au star@BSA was used to bind abundant luminol for labeling the secondary antibody (Ab). Upon the sandwich-typed immunoreactions, the O could react with the introduced luminol on the immunosensor surface to produce strong ECL intensity. With an outstanding linear detection range and a low detection limit of 17 fg/mL, the ECL immunosensor permitted ultrasensitive detection of PCT at a low HO concentration and demonstrated its high application potential in the clinical assay.
一种双信号放大夹心型电化学发光（ECL）免疫传感器被构建用于痕量降钙素原（PCT）的检测。CeO-Au@Pt 由海胆样 Au@Pt 纳米粒子涂覆在 CeO 空心纳米球上组成，被固定在电极表面上以电化学催化 HO 产生大量超氧阴离子（O）。免疫传感器通过将固定在 CeO-Au@Pt 上的捕获抗体与七肽（HWRGWVC）连接来制备，该七肽可以保持抗体的活性。制备的 Au 星@BSA 用于结合大量的鲁米诺以标记二级抗体（Ab）。在三明治型免疫反应中，O 可以与引入的免疫传感器表面上的鲁米诺反应以产生强 ECL 强度。该 ECL 免疫传感器具有出色的线性检测范围和 17 fg/mL 的低检测限，允许在低 HO 浓度下对 PCT 进行超灵敏检测，并在临床分析中显示出其高应用潜力。
