Cui Chen, Liu Naxin, Nian Qiurui, Xia Yuntao, Zhao Liuchuang, Hu Leqian, Bai Hongjuan, Jiang Dechen
College of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, China.
College of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, China.
Talanta. 2025 Feb 1;283:127120. doi: 10.1016/j.talanta.2024.127120. Epub 2024 Oct 28.
Improvement in the sensitivity and accuracy of ECL-based biosensors, to a great extent, depends on the fabrication of high-performance electrochemiluminescence (ECL) sensors. Herein, polyethyleneimine (PEI)-grafted bimetallic ZnNi metal-organic framework (ZnNi-MOF/PEI) nanospheres were synthesized to prepare a L012-based ECL immunosensor for detecting carbohydrate antigen 15-3 (CA15-3). The introduced PEI was utilized not only as a bridging element for L012 luminophore and detection antibody (Ab) immobilization but also as a co-reactant for generating the self-enhanced ZnNi-MOF/PEI-L012@Ab ECL signal probe. Furthermore, Pt nanoparticle-functionalized CeO nanorods (CeO-Pt) featuring high-efficiency electrocatalytic properties, as a co-reaction accelerator, could catalyze HO to generate abundant reactive oxygen species (ROS), further augmenting the ECL signal of ZnNi-MOF/PEI-L012@Ab. In the presence of CA15-3 antigen, the capture antibody (Ab)-conjugated CeO-Pt complex (CeO-Pt@Ab) and ZnNi-MOF/PEI-L012@Ab would get closer to each other, with the formation of a "signal-on" sandwich-type ECL immunosensor. The ZnNi-MOF-assisted ECL biosensor achieved a detection limit of down to 5.75✕10 U mL. This work shows distinguished potential for measuring low levels of biomarkers that may help in the clinical diagnosis of breast cancer.
基于电化学发光（ECL）的生物传感器的灵敏度和准确性的提高在很大程度上取决于高性能电化学发光（ECL）传感器的制备。在此，合成了聚乙烯亚胺（PEI）接枝的双金属ZnNi金属有机框架（ZnNi-MOF/PEI）纳米球，以制备用于检测糖类抗原15-3（CA15-3）的基于L012的ECL免疫传感器。引入的PEI不仅用作L012发光体和检测抗体（Ab）固定的桥连元素，还用作产生自增强的ZnNi-MOF/PEI-L012@Ab ECL信号探针的共反应物。此外，具有高效电催化性能的铂纳米颗粒功能化的CeO纳米棒（CeO-Pt）作为共反应促进剂，可以催化HO产生大量活性氧（ROS），进一步增强ZnNi-MOF/PEI-L012@Ab的ECL信号。在存在CA15-3抗原的情况下，与捕获抗体（Ab）偶联的CeO-Pt复合物（CeO-Pt@Ab）和ZnNi-MOF/PEI-L012@Ab会彼此靠近，形成“信号开启”夹心型ECL免疫传感器。ZnNi-MOF辅助的ECL生物传感器实现了低至5.75×10 U mL的检测限。这项工作在测量低水平生物标志物方面显示出显著潜力，这可能有助于乳腺癌的临床诊断。
