Chenaghlou Salimeh, Khataee Alireza, Jalili Roghayeh, Rashidi Mohammad-Reza, Khalilzadeh Balal, Woo Joo Sang
Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran.
Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran; Institute of Research and Development, Duy Tan University, Da Nang 550000, Viet Nam.
Bioelectrochemistry. 2021 Feb;137:107633. doi: 10.1016/j.bioelechem.2020.107633. Epub 2020 Aug 14.
Gold nanostars (AuNSs) demonstrate an intense electromagnetic field around tip of branches. In this research, we employed AuNSs-enhanced electrochemiluminescence (ECL) emission from graphitic carbon nitride nanosheets (g-CN nanosheets) to detect CD133 peptide as a cancer stem cell membrane biomarker. In this biosensor, the g-CN nanosheets were decorated with AuNSs (AuNSs@g-CN nanosheets). AuNSs@g-CN nanosheets exhibited strong and stable cathodic ECL emission compared to that of pure g-CN nanosheets. The ECL intensity from the AuNSs@g-CN nanosheets was over 30% higher than that of spherical gold nanoparticles (spherical AuNPs) decorated g-CN nanosheets. The additional ECL enhancement of AuNSs was due to the localized surface plasmon resonance (LSPR) effect located around multiple branch tips of AuNSs. The RSD of ECL curves intensities, obtained from successive potential scans for 10 cycles, were less than 4%, indicating the superior stability of the AuNSs@g-CN nanosheets. Under optimum conditions, the ECL intensity of GCE/AuNSs@g-CN nanosheets/anti-CD133 decreased linearly with CD133 peptide concentration in the range of 0.05-100 ng mL. The LOD achieved was 0.257 ng mL (S/N = 3). The applicability of the designed biosensor in real samples was examined through the determination of CD133 peptide in spiked serum samples, from which satisfactory results were obtained.
金纳米星（AuNSs）在其分支尖端周围表现出强烈的电磁场。在本研究中，我们利用金纳米星增强石墨相氮化碳纳米片（g-CN纳米片）的电化学发光（ECL）发射来检测作为癌症干细胞膜生物标志物的CD133肽。在这种生物传感器中，g-CN纳米片用金纳米星进行了修饰（AuNSs@g-CN纳米片）。与纯g-CN纳米片相比，AuNSs@g-CN纳米片表现出强烈且稳定的阴极ECL发射。AuNSs@g-CN纳米片的ECL强度比球形金纳米颗粒（球形AuNPs）修饰的g-CN纳米片高出30%以上。金纳米星额外的ECL增强归因于其多个分支尖端周围的局域表面等离子体共振（LSPR）效应。通过连续10个循环的电位扫描获得的ECL曲线强度的相对标准偏差（RSD）小于4%，表明AuNSs@g-CN纳米片具有优异的稳定性。在最佳条件下，玻碳电极（GCE）/AuNSs@g-CN纳米片/抗CD133的ECL强度随CD133肽浓度在0.05 - 100 ng mL范围内呈线性下降。实现的检测限为0.257 ng mL（信噪比S/N = 3）。通过测定加标血清样品中的CD133肽，检验了所设计生物传感器在实际样品中的适用性，并获得了满意的结果
