Department of Animal Physiology and Physiotherapy, Faculty of Animal Breeding and Biology, Bydgoszcz University of Science and Technology, Mazowiecka 28, 85-004, Bydgoszcz, Poland.
Environ Sci Pollut Res Int. 2022 Feb;29(7):10782-10790. doi: 10.1007/s11356-021-16425-6. Epub 2021 Sep 16.
The aim of the study was to investigate the effects of age and sex on the content of heavy metals (Pb, Cd, Zn and Cu) in the hair, liver and the longissimus lumborum muscle of roe deer (Capreolus capreolus L.). In addition, correlations between heavy metals in individual tested matrices were studied as well as an attempt was made to explain the reasons for their accumulation in specific research matrices. The levels of Zn and Cu were measured using atomic absorption spectroscopy (F-AAS). The levels of Pb and Cd were analysed by means of electrothermal atomic absorption spectroscopy (ET-AAS). In the own research, sex-related statistically significant differences in concentrations of analysed elements were confirmed. Higher levels of the elements were observed in samples collected from does (except for Cd in the liver samples of 6-7-month-old male fawns) but only in some cases, these values were statistically significantly higher. In some cases, age-related differences in the content of individual metals were also confirmed. Age-dependent increased Pb levels in the muscle and liver were reported in 6-7-month-old, <3-4-year-old, <5-6-year-old males. Similar relationships were confirmed for Zn in the bucks' muscles. In the group of does, the increase in heavy metal concentrations with aging was observed for Zn in the muscle and for Pb in the liver. In the other cases, the trend of age-related level changes regarding heavy metals was not clearly demonstrated. Significant positive correlations between Cu and Cd were found for all samples, and significant negative correlations of Cu with Pb were found for the hair and liver. There was a negative correlation of Zn with Cu in all matrices. The study also revealed a negative correlation between Zn and Pb in muscle and a strong significant negative correlation between Zn and Cd in hair and in the liver.
本研究旨在探讨年龄和性别对獐（Capreolus capreolus L.）毛发、肝脏和腰大肌中重金属（Pb、Cd、Zn 和 Cu）含量的影响。此外，还研究了各测试基质中重金属之间的相关性，并试图解释其在特定研究基质中积累的原因。Zn 和 Cu 水平采用原子吸收光谱法（F-AAS）测定，Pb 和 Cd 水平采用电热原子吸收光谱法（ET-AAS）分析。在本研究中，证实了性别与分析元素浓度之间存在统计学上显著的差异。除了 6-7 月龄雄性幼獐肝脏样本中的 Cd 外，母獐样本中各元素的水平均较高，但并非所有情况下这些值均具有统计学显著性差异。在某些情况下，还证实了各年龄段个别金属含量的差异。在 6-7 月龄、<3-4 岁、<5-6 岁雄性獐的肌肉和肝脏中发现 Pb 水平随年龄增长而增加。在公鹿肌肉中也证实了 Zn 存在类似的关系。在母獐组中，随着年龄的增长，肌肉和肝脏中 Zn 和 Pb 的浓度增加。在其他情况下，重金属浓度随年龄变化的趋势并未明显表现出来。在所有样本中均发现 Cu 和 Cd 之间存在显著正相关，Cu 与 Pb 之间在毛发和肝脏中存在显著负相关。在所有基质中 Zn 与 Cu 之间均存在负相关。研究还发现肌肉中 Zn 与 Pb 之间呈负相关，毛发和肝脏中 Zn 与 Cd 之间存在强显著负相关。
