Geng Chang-An, Deng Zhen-Tao, Huang Qian, Xiang Chun-Lei, Chen Ji-Jun
State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China.
Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China.
Nat Prod Bioprospect. 2021 Dec;11(6):643-649. doi: 10.1007/s13659-021-00318-9. Epub 2021 Sep 16.
Ten 3,5-dimethylcoumarins (1-6 and 8‒11) involving six new ones (1-6), together with a known 3-methylcoumarin (7), were isolated from the aerial parts of three Chelonopsis plants, C. praecox, C. odontochila, and C. pseudobracteata. The structures of the new compounds were determined by extensive HRESIMS, 1D and 2D NMR spectroscopic analyses. According to the substitution at C-5, these coumarins were classified into 5-methyl, 5-hydroxymethyl, 5-formyl, and 5-nor types. All the isolates were assayed for their inhibition on α-glucosidase, protein tyrosine phosphatase 1B, and T-cell protein tyrosine phosphatase in vitro.
从三种龙头草属植物，即早花龙头草、齿叶龙头草和假苞片龙头草的地上部分分离得到了10种3,5-二甲基香豆素（1-6和8-11），其中包括6种新化合物（1-6），以及一种已知的3-甲基香豆素（7）。通过广泛的高分辨电喷雾电离质谱、一维和二维核磁共振光谱分析确定了新化合物的结构。根据C-5位的取代情况，这些香豆素被分为5-甲基、5-羟甲基、5-甲酰基和5-降类型。对所有分离物进行了体外抑制α-葡萄糖苷酶、蛋白酪氨酸磷酸酶1B和T细胞蛋白酪氨酸磷酸酶活性的测定。
