Passarelli-Araujo Hemanoel, Jacobs Sarah H, Franco Glória R, Venancio Thiago M
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil; Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil.
Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil.
Genomics. 2021 Nov;113(6):3762-3773. doi: 10.1016/j.ygeno.2021.09.008. Epub 2021 Sep 13.
The Pseudomonas putida group comprises strains with biotechnological and clinical relevance. P. alloputida was proposed as a new species and highlighted the misclassification of P. putida. Nevertheless, the population structure of P. alloputida remained unexplored. We retrieved 11,025 Pseudomonas genomes and used P. alloputida Kh7 to delineate the species. The P. alloputida population structure comprises at least 7 clonal complexes (CCs). Clinical isolates are mainly found in CC4 and acquired resistance genes are present at low frequency in plasmids. Virulence profiles support the potential of CC7 members to outcompete other plant or human pathogens through a type VI secretion system. Finally, we found that horizontal gene transfer had an important role in shaping the ability of P. alloputida to bioremediate aromatic compounds such as toluene. Our results provide the grounds to understand P. alloputida genetic diversity and its potential for biotechnological applications.
恶臭假单胞菌群包含具有生物技术和临床相关性的菌株。别恶臭假单胞菌被提议作为一个新物种，并凸显了恶臭假单胞菌的错误分类。然而，别恶臭假单胞菌的种群结构仍未得到探索。我们检索了11025个假单胞菌基因组，并使用别恶臭假单胞菌Kh7来界定该物种。别恶臭假单胞菌的种群结构至少包含7个克隆复合体（CCs）。临床分离株主要存在于CC4中，获得性耐药基因在质粒中的出现频率较低。毒力谱支持CC7成员通过VI型分泌系统胜过其他植物或人类病原体的潜力。最后，我们发现水平基因转移在塑造别恶臭假单胞菌对甲苯等芳香族化合物进行生物修复的能力方面发挥了重要作用。我们的结果为理解别恶臭假单胞菌的遗传多样性及其在生物技术应用中的潜力提供了依据。
