中国仓鼠胸苷激酶基因的生长阶段依赖性和腺病毒5反应性表达的遗传决定因素包含在胸苷激酶mRNA序列中。
Genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the Chinese hamster thymidine kinase gene are contained within thymidine kinase mRNA sequences.
作者信息
Lewis J A, Matkovich D A
出版信息
Mol Cell Biol. 1986 Jun;6(6):2262-6. doi: 10.1128/mcb.6.6.2262-2266.1986.
Abstract
We have constructed a chimeric thymidine kinase (TK) minigene, pHe delta 6Ha, which combines the complete coding and 3' noncoding regions of a Chinese hamster TK cDNA with the promoter region and 5' untranslated region of the TK gene of herpes simplex virus type 1. We have transformed rat 4 cells to Tk+ with this gene and analyzed the pattern of TK gene expression in these transformants under various conditions of in vitro cell culture. We find that TK gene expression in these Tk+ transformants is growth phase dependent, responsive to adenovirus 5 infection, and indistinguishable in character under a variety of cell culture conditions from the pattern of TK gene expression in rat 4 cells transformed to Tk+ with the genomic Chinese hamster TK gene clone lambda HaTK.5. We are led to the conclusion that the genetic elements which mediate growth phase-dependent TK gene expression are contained entirely within the sequences of the mature cytoplasmic hamster TK mRNA.
摘要
我们构建了一个嵌合胸苷激酶（TK）小基因pHeδ6Ha，它将中国仓鼠TK cDNA的完整编码区和3'非编码区与单纯疱疹病毒1型TK基因的启动子区和5'非翻译区结合在一起。我们用这个基因将大鼠4细胞转化为Tk+，并在各种体外细胞培养条件下分析了这些转化体中TK基因的表达模式。我们发现，这些Tk+转化体中的TK基因表达依赖于生长阶段，对腺病毒5感染有反应，并且在多种细胞培养条件下，其特征与用基因组中国仓鼠TK基因克隆λHaTK.5转化为Tk+的大鼠4细胞中TK基因的表达模式无法区分。我们得出结论，介导生长阶段依赖性TK基因表达的遗传元件完全包含在成熟的细胞质仓鼠TK mRNA序列中。
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Genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the Chinese hamster thymidine kinase gene are contained within thymidine kinase mRNA sequences.中国仓鼠胸苷激酶基因的生长阶段依赖性和腺病毒5反应性表达的遗传决定因素包含在胸苷激酶mRNA序列中。
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