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长链非编码RNA FEZF1-AS1通过靶向微小RNA-1254调控宫颈癌的生物学行为。

lncRNA FEZF1-AS1 regulates biological behaviors of cervical cancer by targeting miRNA-1254.

作者信息

Liang Miao, Li Yongkang, Dai Tingting, Chen Cheng

机构信息

Department of gynaecology and obstetrics Chongqing General Hospital University of Chinese Academy of Sciences Chongqing China.

出版信息

Food Sci Nutr. 2021 Jul 14;9(9):4722-4737. doi: 10.1002/fsn3.2315. eCollection 2021 Sep.

DOI:10.1002/fsn3.2315
PMID:34531986
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8441442/
Abstract

AIM

The purpose of this research was to evaluate lncRNA FEZF1-AS1 in cervical cancer development and clinical significance.

MATERIALS AND METHODS

Collecting cervical cancer tissues, measuring FEZF1-AS1 expression, and analysis correlation between FEZF1-AS1 and prognosis. In cell vitro study, using MTT assay to measure cell proliferation, evaluating cell apoptosis by flow cytometry, measuring cell invasion and migration by Transwell and wound healing assay; lncRNA FEZF1-AS1 and miR-1254 gene expressions were evaluated by RT-qPCR assay; relative protein (Smurf1, E-cadherin, Vimentin, N-cadherin, AKT, p-AKT, c-Myc, and ZEB1) expressions were measured by Western blot assay. The correlation among FEZF1-AS1, miR-1254, and Smurf1 were analysis by dual luciferase reporter gene assay.

RESULTS

By clinical analysis, lncRNA FEZF1-AS1 was high expression in cervical cancer tissues and high expression was closely correlated with poor prognosis in cervical cancer patients. In vitro study, the SiHa and HeLa cell biologically including cell proliferation, migration, and invasion of si-FEZF1-AS1 group which knockdown lncRNA FEZF1-AS1 were significantly depressed ( < .001, respectively). However, with miR-1254 expression inhibiting, the cell biological activities were significantly increased in si-FEZF1-AS1+miRNA inhibitor groups ( < .001, respectively).

CONCLUSION

lncRNA FEZF1-AS1 might be an oncological role in cervical cancer; lncRNA FEZF1-AS1 knockdown had antitumor effects with miR-1254 activating in cervical cancer by in vitro study.

摘要

目的

本研究旨在评估长链非编码RNA FEZF1-AS1在宫颈癌发生发展中的作用及临床意义。

材料与方法

收集宫颈癌组织，检测FEZF1-AS1表达，并分析FEZF1-AS1与预后的相关性。在体外细胞研究中，采用MTT法检测细胞增殖，通过流式细胞术评估细胞凋亡，利用Transwell和伤口愈合试验检测细胞侵袭和迁移；采用RT-qPCR法检测长链非编码RNA FEZF1-AS1和miR-1254基因表达；采用蛋白质印迹法检测相关蛋白（Smurf1、E-钙黏蛋白、波形蛋白、N-钙黏蛋白、AKT、p-AKT、c-Myc和ZEB1）表达。通过双荧光素酶报告基因试验分析FEZF1-AS1、miR-1254和Smurf1之间的相关性。

结果

临床分析显示，长链非编码RNA FEZF1-AS1在宫颈癌组织中高表达，且高表达与宫颈癌患者预后不良密切相关。体外研究表明，敲低长链非编码RNA FEZF1-AS1的SiHa和HeLa细胞生物学行为，包括细胞增殖、迁移和侵袭，均显著受到抑制（分别为P<0.001）。然而，抑制miR-1254表达后，si-FEZF1-AS1+miRNA抑制剂组的细胞生物学活性显著增加（分别为P<0.001）。

结论

长链非编码RNA FEZF1-AS1在宫颈癌中可能发挥致癌作用；体外研究表明，敲低长链非编码RNA FEZF1-AS1并激活miR-1254在宫颈癌中具有抗肿瘤作用。

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