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结合化学计量学的人工仿生味觉传感器用于牛肉掺假的快速检测。

Artificial bionic taste sensors coupled with chemometrics for rapid detection of beef adulteration.

作者信息

Lu Biao, Han Fangkai, Aheto Joshua H, Rashed Marwan M A, Pan Zhenggao

机构信息

School of Information and Engineering Suzhou University Suzhou China.

School of Biological and Food Engineering Suzhou University Suzhou China.

出版信息

Food Sci Nutr. 2021 Jul 29;9(9):5220-5228. doi: 10.1002/fsn3.2494. eCollection 2021 Sep.

Abstract

The purpose of this study was to investigate the potential of taste sensors coupled with chemometrics for rapid determination of beef adulteration. A total of 228 minced meat samples were prepared and analyzed via raw ground beef mixed separately with chicken, duck, and pork in the range of 0 ~ 50% by weight at 10% intervals. Total sugars, protein, fat, and ash contents were also measured to validate the differences between raw meats. For sensing the water-soluble chemicals in the meats, an electronic tongue based on multifrequency large-amplitude pulses and six metal electrodes (platinum, gold, palladium, tungsten, titanium, and silver) was employed. Fisher linear discriminant analysis (Fisher LDA) and extreme learning machine (ELM) were used to model the identification of raw and the adulterated meats. While an adulterant was detected, the level of adulteration was predicted using partial least squares (PLS) and ELM and the results compared. The results showed that superior recognition models derived from ELM were obtained, as the recognition rates for the independent samples in different meat groups were all over 90%; ELM models were more precisely than PLS models for prediction of the adulteration levels of beef mixed with chicken, duck, and pork, with root mean squares error for the independent samples of 0.33, 0.18, and 0.38% and coefficients of variance of 0.914, 0.956, and 0.928, respectively. The results suggested that taste sensors combined with ELM could be useful in the rapid detection of beef adulterated with other meats.

摘要

本研究的目的是探究味觉传感器结合化学计量学用于快速测定牛肉掺假情况的潜力。总共制备了228个碎肉样品,并通过将生牛肉末分别与鸡肉、鸭肉和猪肉按重量在0至50%范围内以10%的间隔混合进行分析。还测量了总糖、蛋白质、脂肪和灰分含量,以验证生肉之间的差异。为了检测肉中的水溶性化学物质,使用了基于多频大幅度脉冲和六个金属电极(铂、金、钯、钨、钛和银)的电子舌。采用费舍尔线性判别分析(Fisher LDA)和极限学习机(ELM)对生肉和掺假肉的识别进行建模。当检测到掺假物时,使用偏最小二乘法(PLS)和ELM预测掺假水平,并比较结果。结果表明,基于ELM获得了优越的识别模型,因为不同肉类组中独立样本的识别率均超过90%;对于预测与鸡肉、鸭肉和猪肉混合的牛肉的掺假水平,ELM模型比PLS模型更精确,独立样本的均方根误差分别为0.33%、0.18%和0.38%,变异系数分别为0.914、0.956和0.928。结果表明,味觉传感器结合ELM可用于快速检测掺有其他肉类的牛肉。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f623/8441491/4234cd11fa47/FSN3-9-5220-g001.jpg

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