Zhao Xueyan, Wang Ziyu, Wu Yulin, Cai Huiming
School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, China.
Nanjing Nuoyuan Medical Devices Co., Ltd., Nanjing, China.
Ann Transl Med. 2021 Aug;9(15):1238. doi: 10.21037/atm-21-3050.
Surgical operation plays an important role in the treatment of cancer. The success of the operation lies in the complete removal of the primary and disseminated tumor tissue while preserving the normal tissue. The development of optical molecular image navigation technology has provided a new option for intraoperative tumor visualization. In this study, a fluorescence imaging navigation system was used to detect the diameter of mice tumors and provide experimental evidence for the further development of digital diagnosis and treatment equipment.
The minimum detection concentration in vitro of the fluorescence imaging navigation system for indocyanine green (ICG) was first detected, then 120 female Institute of Cancer Research (ICR) mice and 120 female BALB/c nude mice were randomly divided into three groups by weight, high-dose (H, 4 mg/kg), middle-dose (M, 2 mg/kg), and low-dose (L, 1 mg/kg) groups of ICG solution. After inoculating solid tumors, high, medium, and low doses of ICG were injected via the tail vein, and the tumor diameter was measured by a fluorescence imaging navigation system and vernier caliper within 24 hours of injection.
The minimum detectable diameter of the system could reach 0.2 mm compared with the vernier caliper, and the actual measurement error was within 0.2 mm.
A fluorescence imaging navigation system has high accuracy and sensitivity in the application of tumor detection, which may assist the clinical diagnosis and treatment of tumors.
外科手术在癌症治疗中发挥着重要作用。手术的成功在于在保留正常组织的同时完全切除原发性和播散性肿瘤组织。光学分子影像导航技术的发展为术中肿瘤可视化提供了新的选择。在本研究中，使用荧光成像导航系统检测小鼠肿瘤直径，为数字诊疗设备的进一步研发提供实验依据。
首先检测荧光成像导航系统对吲哚菁绿（ICG）的体外最低检测浓度，然后将120只雌性癌症研究机构（ICR）小鼠和120只雌性BALB/c裸鼠按体重随机分为三组，分别注射高剂量（H，4mg/kg）、中剂量（M，2mg/kg）和低剂量（L，1mg/kg）的ICG溶液。接种实体瘤后，经尾静脉注射高、中、低剂量的ICG，在注射后24小时内用荧光成像导航系统和游标卡尺测量肿瘤直径。
与游标卡尺相比，该系统的最小可检测直径可达0.2mm，实际测量误差在0.2mm以内。
荧光成像导航系统在肿瘤检测应用中具有较高的准确性和灵敏度，可能有助于肿瘤的临床诊断和治疗。
