Kreplin C M, Ruhnke H L, Miller R B, Doig P A
Department of Pathology, Ontario Veterinary College, University of Guelph.
Can J Vet Res. 1987 Oct;51(4):440-3.
To determine the influence of Ureaplasma diversum on bovine fertility 11 uninfected virgin heifers with normal ovarian cyclic activity were randomly allocated to test or control groups. At a synchronized estrus, five test heifers were given an intrauterine broth inoculum containing 1.09 x 10(8) to 1.4 x 10(9) colony forming units of U. diversum and six control animals were infused with sterile ureaplasma broth medium. All animals were artificially inseminated within one hour of infusion. Pregnancy was diagnosed in one of five test heifers and all of six controls by serum progesterone concentrations measured to 25 days postinsemination. The difference in pregnancy rates between the two groups was statistically significant (p = 0.0152). It was concluded that under the conditions of this experiment U. diversum is capable of causing infertility in cattle.
为确定差异脲原体对奶牛繁殖力的影响,将11头卵巢周期活动正常的未感染处女小母牛随机分为试验组和对照组。在同步发情时,给5头试验小母牛子宫内接种含有1.09×10⁸至1.4×10⁹个差异脲原体菌落形成单位的肉汤,给6头对照动物注入无菌脲原体肉汤培养基。所有动物在注入后1小时内进行人工授精。通过测定授精后25天的血清孕酮浓度,在5头试验小母牛中有1头、6头对照小母牛全部怀孕。两组之间的怀孕率差异具有统计学意义(p = 0.0152)。得出结论,在本实验条件下,差异脲原体能够导致奶牛不孕。