Faculty of Pharmacy, Middle East University, Amman, Jordan.
Eur Rev Med Pharmacol Sci. 2021 Sep;25(17):5507-5510. doi: 10.26355/eurrev_202109_26661.
The paper presents the antibacterial activity of silver nanoparticles (AgNPs) when conjugated with Levofloxacin. The AgNPs used in this study were synthesized from silver nitrate using sodium borohydride as a reducing agent.
Levofloxacin activity was determined by minimum inhibitory concentrations (MICs) and also the erythrocyte hemolytic assay determined the capability of conjugation to cause hemolysis in human erythrocyte.
The synthesis of levofloxacin-AgNP conjugates was confirmed by ultraviolet/visible (UV/vis) spectroscopy. A peak absorption value between 400-450 nm for the extract and the color change to dark brown were corresponding to the plasmon absorbance of AgNPs. On the other hand, levofloxacin-AgNPs could be effective against methicillin-resistant Staphylococcus aureus (MRSA). The MICs of levofloxacin and levofloxacin-AgNPs were 12 and 10 µM, respectively.
These findings indicated that levofloxacin-AgNPs had an effective bactericidal activity against the bacterial MRSAs. This conjugation appeared to inhibit bacterial adaptive capabilities, which leads to inhibition of bacterial resistance.
本文介绍了银纳米粒子（AgNPs）与左氧氟沙星结合时的抗菌活性。本研究中使用的 AgNPs 是通过使用硼氢化钠作为还原剂从硝酸银合成的。
通过最小抑菌浓度（MICs）测定左氧氟沙星的活性，同时通过红细胞溶血试验测定结合物导致人红细胞溶血的能力。
通过紫外/可见（UV/vis）光谱证实了左氧氟沙星-AgNP 缀合物的合成。提取物的 400-450nm 之间的峰值吸收值和颜色变为深棕色与 AgNPs 的等离子体吸收相对应。另一方面，左氧氟沙星-AgNPs 可有效对抗耐甲氧西林金黄色葡萄球菌（MRSA）。左氧氟沙星和左氧氟沙星-AgNPs 的 MIC 值分别为 12 和 10µM。
这些发现表明，左氧氟沙星-AgNPs 对细菌 MRSAs 具有有效的杀菌活性。这种缀合似乎抑制了细菌的适应能力，从而抑制了细菌的耐药性。
