School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Veterinary Bio-tech Key Laboratory, Shanghai 200240, China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Int J Biol Macromol. 2021 Nov 30;191:71-78. doi: 10.1016/j.ijbiomac.2021.09.027. Epub 2021 Sep 14.
In this study, cellulose nanocrystals (CNC) as functional cross-linker and Pickering emulsifier was used to stabilize Lysozyme (Lys) encapsulated in quaternary ammonium chitosan nanoparticles (QC NPs) via ionic gelation method. Physicochemical, structural, and antibacterial properties of the CNC stabilized Lys loaded QC NPs were also evaluated. Particle size, particle size distribution, Zeta potential (ZP), and spectroscopic analyses showed the successful encapsulation of Lys. Antibacterial activity of NPs against Staphylococcus aureus and Vibrio parahaemolyticus was investigated on the basis of inhibition zone (IZ), minimum inhibitory concentration (MIC), and minimum bacterial concentration (MBC). MIC and MBC of CNC stabilized Lys loaded HQC NPs against S. aureus were 0.094 and 0.188 while corresponding values for CNC stabilized Lys loaded LQC NPs V. parahaemolyticus were 0.156 and 0.312 mg/mL, respectively. Therefore, CNC stabilized Lys loaded QC NPs have potential implications in the food industry for food preservation and packaging.
在这项研究中，纤维素纳米晶体（CNC）被用作功能性交联剂和 Pickering 乳化剂，通过离子凝胶法稳定壳聚糖季铵盐纳米颗粒（QC NPs）中包封的溶菌酶（Lys）。还评估了 CNC 稳定的负载 Lys 的 QC NPs 的物理化学、结构和抗菌性能。颗粒大小、颗粒大小分布、Zeta 电位（ZP）和光谱分析表明 Lys 成功被包封。根据抑菌圈（IZ）、最小抑菌浓度（MIC）和最小细菌浓度（MBC），研究了 NPs 对金黄色葡萄球菌和副溶血弧菌的抗菌活性。CNC 稳定的负载 Lys 的 HQC NPs 对金黄色葡萄球菌的 MIC 和 MBC 分别为 0.094 和 0.188，而 CNC 稳定的负载 Lys 的 LQC NPs 对副溶血弧菌的 MIC 和 MBC 分别为 0.156 和 0.312 mg/mL。因此，CNC 稳定的负载 Lys 的 QC NPs 在食品工业中具有用于食品保鲜和包装的潜力。
