杨树中 MYB 相关转录因子家族的全基因组分析及其对非生物胁迫因子的响应

Genome-wide analysis of the MYB-related transcription factor family and associated responses to abiotic stressors in Populus.

机构信息

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China; National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China; Key Laboratory of State Forestry and Grassland Administration on Tropical Forestry Research, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China.

出版信息

Int J Biol Macromol. 2021 Nov 30;191:359-376. doi: 10.1016/j.ijbiomac.2021.09.042. Epub 2021 Sep 14.

DOI:10.1016/j.ijbiomac.2021.09.042

PMID:34534587

Abstract

MYB proteins are one of the most abundant transcription factor families in the plant kingdom. Evidence has increasingly revealed that MYB-related proteins function in diverse plant biological processes. However, little is known about the genome-wide characterization and functions of MYB-related proteins in Populus, an important model and commercial tree species. In this study, 152 PtrMYBRs were identified and unevenly located on 19 Populus chromosomes. A phylogenetic analysis divided them into six major subgroups, supported by conserved gene organization, consensus motifs, and protein domain architecture. Promoter assessment and gene ontology classification results indicated that the MYB-related family is likely involved in plant development and responses to various environmental stressors. The Populus MYB-related family members showed various expression patterns in different tissues and stress conditions, implying their crucial roles in the development and stress responses in Populus. Co-expression analyses revealed that Populus MYB-related genes might participate in the regulation of antioxidant defense system and various signaling pathways in response to stress. The three-dimensional structures of different subgroup of Populus MYB-related proteins further provided functional information at the proteomic level. These findings provide valuable information for a prospective functional dissection of MYB-related proteins and genetic improvement of Populus.

摘要

MYB 蛋白是植物界中最丰富的转录因子家族之一。越来越多的证据表明，MYB 相关蛋白在植物的多种生物学过程中发挥作用。然而，关于 MYB 相关蛋白在杨树这一重要的模式和商业树种中的全基因组特征和功能，人们知之甚少。本研究在杨树中鉴定到 152 个 PtrMYBRs，并不均匀地分布在 19 条杨树染色体上。系统发育分析将它们分为 6 个主要亚群，这得到了保守的基因组织、共有基序和蛋白结构域结构的支持。启动子评估和基因本体分类结果表明，MYB 相关家族可能参与植物发育和对各种环境胁迫的响应。杨树 MYB 相关家族成员在不同组织和胁迫条件下表现出不同的表达模式，这表明它们在杨树的发育和胁迫响应中起着至关重要的作用。共表达分析表明，杨树 MYB 相关基因可能参与抗氧化防御系统的调节以及对胁迫的各种信号通路的调节。不同亚群的杨树 MYB 相关蛋白的三维结构进一步提供了蛋白质组水平的功能信息。这些发现为 MYB 相关蛋白的功能解析和杨树的遗传改良提供了有价值的信息。