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. 中MYB相关亚家族基因的特征与表达分析

Characteristics and Expression Analysis of the MYB-Related Subfamily Gene in .

作者信息

Zhu Yongjie, Deng Yuzheng, Yao Yandong, Yao Kangding, Pan Xuejuan, Wu Xuetong, Liu Zhiya, Zhang Jitao, Su Wanyi, Liao Weibiao

机构信息

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.

出版信息

Int J Mol Sci. 2024 Nov 29;25(23):12854. doi: 10.3390/ijms252312854.

Abstract

MYB-related transcription factors (TFs) subfamily is a subfamily of MYB TFs, which are mainly involved in plant secondary metabolism, growth and development, and stress response. To explore the function of MYB-related subfamily genes in , this study systematically analyzed characters of the MYB-related subfamily members in . with bioinformatic analysis using the genomic data of . and investigated their expression characteristics using quantitative real-time PCR (qRT-PCR). The results show that 100 MYB-related proteins were identified in . . Proteins are mainly found in the nucleus. Chromosome localization revealed that all MYB-related genes are mapped to seven chromosomes and are distributed in clusters. Collinear analysis shows that 13 pairs of MYB-related genes had a collinear relationship, indicating may have evolved its MYB-related subfamily gene through fragment duplication. The analysis of motifs and conserved domains shows that Motif 3 is the most conserved motif. There are numerous ABA and MeJA response elements in MYB-related genes. ABA and MeJA treatments significantly shortened the vase life of , while the flower diameter on day 3 was the largest, suggesting that ABA and MeJA might induce MYB-related gene expression during cut flower senescence. The expression of MYB-related genes is tissue specific, most of which show the highest expression levels in petals. Notably, among six plant growth regulator treatments, ABA treatment significantly increased expression in , suggesting that may be a crucial gene for ABA response. This study provides a reference for further research on the function of MYB-related genes in . .

摘要

MYB相关转录因子(TFs)亚家族是MYB转录因子的一个亚家族,主要参与植物次生代谢、生长发育及胁迫响应。为探究MYB相关亚家族基因在[具体植物名称未给出]中的功能,本研究利用[具体植物名称未给出]的基因组数据,通过生物信息学分析系统地分析了MYB相关亚家族成员的特征,并采用实时定量PCR(qRT-PCR)研究了它们的表达特性。结果表明,在[具体植物名称未给出]中鉴定出100个MYB相关蛋白。这些蛋白主要存在于细胞核中。染色体定位显示,所有MYB相关基因均定位到7条染色体上并成簇分布。共线性分析表明,有13对MYB相关基因存在共线性关系,这表明[具体植物名称未给出]可能通过片段复制进化出其MYB相关亚家族基因。基序和保守结构域分析表明,基序3是最保守的基序。MYB相关基因中有大量脱落酸(ABA)和茉莉酸甲酯(MeJA)响应元件。ABA和MeJA处理显著缩短了[具体植物名称未给出]的瓶插寿命,而第3天的花径最大,这表明ABA和MeJA可能在切花衰老过程中诱导MYB相关基因表达。MYB相关基因的表达具有组织特异性,其中大部分在花瓣中表达水平最高。值得注意的是,在六种植物生长调节剂处理中,ABA处理显著增加了[具体植物名称未给出]中的[具体基因名称未给出]表达,这表明[具体基因名称未给出]可能是ABA响应的关键基因。本研究为进一步研究[具体植物名称未给出]中MYB相关基因的功能提供了参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24f7/11640956/fdfb8668904d/ijms-25-12854-g001.jpg

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