Zhu Yongjie, Deng Yuzheng, Yao Yandong, Yao Kangding, Pan Xuejuan, Wu Xuetong, Liu Zhiya, Zhang Jitao, Su Wanyi, Liao Weibiao
College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Int J Mol Sci. 2024 Nov 29;25(23):12854. doi: 10.3390/ijms252312854.
MYB-related transcription factors (TFs) subfamily is a subfamily of MYB TFs, which are mainly involved in plant secondary metabolism, growth and development, and stress response. To explore the function of MYB-related subfamily genes in , this study systematically analyzed characters of the MYB-related subfamily members in . with bioinformatic analysis using the genomic data of . and investigated their expression characteristics using quantitative real-time PCR (qRT-PCR). The results show that 100 MYB-related proteins were identified in . . Proteins are mainly found in the nucleus. Chromosome localization revealed that all MYB-related genes are mapped to seven chromosomes and are distributed in clusters. Collinear analysis shows that 13 pairs of MYB-related genes had a collinear relationship, indicating may have evolved its MYB-related subfamily gene through fragment duplication. The analysis of motifs and conserved domains shows that Motif 3 is the most conserved motif. There are numerous ABA and MeJA response elements in MYB-related genes. ABA and MeJA treatments significantly shortened the vase life of , while the flower diameter on day 3 was the largest, suggesting that ABA and MeJA might induce MYB-related gene expression during cut flower senescence. The expression of MYB-related genes is tissue specific, most of which show the highest expression levels in petals. Notably, among six plant growth regulator treatments, ABA treatment significantly increased expression in , suggesting that may be a crucial gene for ABA response. This study provides a reference for further research on the function of MYB-related genes in . .
MYB相关转录因子(TFs)亚家族是MYB转录因子的一个亚家族,主要参与植物次生代谢、生长发育及胁迫响应。为探究MYB相关亚家族基因在[具体植物名称未给出]中的功能,本研究利用[具体植物名称未给出]的基因组数据,通过生物信息学分析系统地分析了MYB相关亚家族成员的特征,并采用实时定量PCR(qRT-PCR)研究了它们的表达特性。结果表明,在[具体植物名称未给出]中鉴定出100个MYB相关蛋白。这些蛋白主要存在于细胞核中。染色体定位显示,所有MYB相关基因均定位到7条染色体上并成簇分布。共线性分析表明,有13对MYB相关基因存在共线性关系,这表明[具体植物名称未给出]可能通过片段复制进化出其MYB相关亚家族基因。基序和保守结构域分析表明,基序3是最保守的基序。MYB相关基因中有大量脱落酸(ABA)和茉莉酸甲酯(MeJA)响应元件。ABA和MeJA处理显著缩短了[具体植物名称未给出]的瓶插寿命,而第3天的花径最大,这表明ABA和MeJA可能在切花衰老过程中诱导MYB相关基因表达。MYB相关基因的表达具有组织特异性,其中大部分在花瓣中表达水平最高。值得注意的是,在六种植物生长调节剂处理中,ABA处理显著增加了[具体植物名称未给出]中的[具体基因名称未给出]表达,这表明[具体基因名称未给出]可能是ABA响应的关键基因。本研究为进一步研究[具体植物名称未给出]中MYB相关基因的功能提供了参考。