Tanaka T, Hachinoe M, Kubodera A
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Steroids. 1987 Jun;49(6):523-30. doi: 10.1016/0039-128x(87)90092-4.
The hapten of deoxycorticosterone sulfate was synthesized and linked to the carrier protein through the C-4 position on the steroid nucleus. The antisera raised against this antigen in guinea pigs exhibited high affinity (Ka = 1.86 x 10(9) M-1) and excellent specificity for deoxycorticosterone sulfate. It was found that deoxycorticosterone sulfate can be determined in the range of 50-5000 pg, and this radioimmunoassay in plasma shows a coefficient of variation (CV) less than 10%.
合成了硫酸脱氧皮质酮的半抗原，并通过甾体核上的C-4位将其与载体蛋白相连。在豚鼠中针对该抗原产生的抗血清对硫酸脱氧皮质酮表现出高亲和力（Ka = 1.86 x 10⁹ M⁻¹）和优异的特异性。发现硫酸脱氧皮质酮的测定范围为50 - 5000 pg，血浆中的这种放射免疫测定法显示变异系数（CV）小于10%。
