用于硫酸雌三醇3放射免疫测定的特异性抗血清。

Specific antisera for the radioimmunoassay of estriol 3-sulfate.

作者信息

Tanaka T, Suguro N, Kubodera A

出版信息

Steroids. 1984 Mar;43(3):235-42. doi: 10.1016/0039-128x(84)90042-4.

Abstract

Antisera were raised in male guinea pigs against 6-oxoestriol 3-sulfate O-carboxymethyloxime-bovine serum albumin (BSA) conjugate. The antisera to this antigen exhibited high affinity (Ka=4.7 X 10(9)M-1) and excellent specificity for estriol 3-sulfate, showing slight cross-reactions (less than 0.43%) with other estrogen sulfates, and no cross-reactivities with free estrogens and other steroids (less than 0.01%) except cholesterol sulfate (0.22%). A standard curve using [6, 7-3H]-estriol 3-sulfate as the radioactive ligand showed high sensitivity in the range of 10-1000 pg estriol 3-sulfate.

摘要

在雄性豚鼠体内制备了针对6-氧代雌三醇3-硫酸酯O-羧甲基肟-牛血清白蛋白（BSA）偶联物的抗血清。该抗原的抗血清对雌三醇3-硫酸酯表现出高亲和力（Ka = 4.7×10⁹M⁻¹）和优异的特异性，与其他雌激素硫酸盐有轻微交叉反应（小于0.43%），除硫酸胆固醇（0.22%）外，与游离雌激素和其他类固醇无交叉反应（小于0.01%）。以[6,7-³H]-雌三醇3-硫酸酯作为放射性配体的标准曲线在10 - 1000 pg雌三醇3-硫酸酯范围内显示出高灵敏度。