Habibinava Fatemeh, Zolfaghari Mohammad Reza, Zargar Mohsen, Shahrbabak Salehe Sabouri, Soleimani Mohammad
Department of Microbiology, Faculty of Basic Sciences, Qom Branch, Islamic Azad University, Qom, Iran.
Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Iran J Microbiol. 2021 Apr;13(2):225-234. doi: 10.18502/ijm.v13i2.5984.
Multi-drug-resistant is associated with various infectious diseases that cannot be easily treated by antibiotics. However, bacteriophages have potential therapeutic applications in the control of multi-drug-resistant bacteria. In this study, we aimed to isolate and characterize of a lytic bacteriophage that can lyse specifically the multi-drug-resistant (MDR)
Lytic bacteriophage was isolated from Qaem hospital wastewater and characterized morphologically and genetically. Next-generation sequencing was used to complete genome analysis of the isolated bacteriophage.
Based on the transmission electron microscopy feature, the isolated bacteriophage (vB-Ea-5) belongs to the family . vB-Ea-5 had a latent period of 25 minutes, a burst size of 13 PFU/ml, and a burst time of 40 min. Genome sequencing revealed that vB-Ea-5 has a 135324 bp genome with 41.41% GC content. The vB-Ea-5 genome codes 212 ORFs 90 of which were categorized into several functional classes such as DNA replication and modification, transcriptional regulation, packaging, structural proteins, and a host lysis protein (Holin). No antibiotic resistance and toxin genes were detected in the genome. SDS-PAGE of vB-Ea-5 proteins exhibited three major and four minor bands with a molecular weight ranging from 18 to 50 kD.
Our study suggests vB-Ea-5 as a potential candidate for phage therapy against MDR infections.
多重耐药性与多种难以用抗生素轻易治疗的传染病相关。然而，噬菌体在控制多重耐药菌方面具有潜在的治疗应用价值。在本研究中，我们旨在分离并鉴定一种能够特异性裂解多重耐药菌的裂解性噬菌体。
从卡姆医院废水中分离出裂解性噬菌体，并对其进行形态学和遗传学特征分析。采用下一代测序技术完成对分离出的噬菌体的基因组分析。
基于透射电子显微镜特征，分离出的噬菌体（vB-Ea-5）属于 科。vB-Ea-5 的潜伏期为 25 分钟，裂解量为 13 PFU/ml，裂解时间为 40 分钟。基因组测序显示，vB-Ea-5 具有一个 135324 bp 的基因组，GC 含量为 41.41%。vB-Ea-5 基因组编码 212 个开放阅读框（ORF），其中 90 个可归类为几个功能类别，如 DNA 复制与修饰、转录调控、包装、结构蛋白和一种宿主裂解蛋白（穿孔素）。基因组中未检测到抗生素抗性和毒素基因。vB-Ea-5 蛋白的 SDS-PAGE 显示出三条主要带和四条次要带，分子量范围为 18 至 50 kD。
我们的研究表明 vB-Ea-5 是针对多重耐药菌感染进行噬菌体治疗的潜在候选者。
