利用负压吸收法（TUSP）从极化上皮细胞中物理去除中体残余物。
Physical Removal of the Midbody Remnant from Polarised Epithelial Cells Using Take-Up by Suction Pressure (TUSP).
作者信息
Bernabé-Rubio Miguel, Gershlick David C, Alonso Miguel A
机构信息
Department of Cell Biology and Immunology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, Madrid, Spain.
Cell Biology and Neurobiology Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, USA.
出版信息
Bio Protoc. 2017 Apr 20;7(8):e2244. doi: 10.21769/BioProtoc.2244.
In polarised epithelial cells the midbody forms at the apical cell surface during cytokinesis. Once severed, the midbody is inherited by one of the daughter cells remaining tethered to the apical plasma membrane where it participates in non-cytokinetic processes, such as primary ciliogenesis. Here, we describe a novel method to physically remove the midbody remnant from cells and assess the possible effects caused by its loss (Bernabé- Rubio , 2016 ).
在极化上皮细胞中，中体在胞质分裂期间于顶端细胞表面形成。一旦切断，中体由保留附着于顶端质膜的其中一个子细胞继承，在那里它参与非胞质分裂过程，如初级纤毛发生。在此，我们描述了一种从细胞中物理去除中体残余物并评估其缺失可能造成的影响的新方法（Bernabé - Rubio，2016）。
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