固定出芽酵母细胞中肌动蛋白细胞骨架的成像。
Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells.
机构信息
Department of Pathology and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, NY, USA.
Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, USA.
出版信息
Methods Mol Biol. 2022;2364:81-100. doi: 10.1007/978-1-0716-1661-1_4.
Budding yeast, Saccharomyces cerevisiae, is an appealing model organism to study the organization and function of the actin cytoskeleton. With the advent of techniques to perform high-resolution, multidimensional analysis of the yeast cell, imaging of yeast has emerged as an important tool for research on the cytoskeleton. This chapter describes techniques and approaches for visualizing the actin cytoskeleton in fixed yeast cells with wide-field and super-resolution fluorescence microscopy.
budding 酵母，酿酒酵母，是研究肌动蛋白细胞骨架的组织和功能的一种很有吸引力的模式生物。随着能够对酵母细胞进行高分辨率、多维分析的技术的出现，酵母成像已成为研究细胞骨架的重要工具。本章介绍了使用宽场和超分辨率荧光显微镜固定酵母细胞中肌动蛋白细胞骨架的可视化技术和方法。
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