Institute for Plant Genetics, Faculty of Natural Sciences, Leibniz Universität Hannover, Herrenhäuser Str. 2, D-30419 Hannover, Germany.
Phytochemistry. 2011 Jul;72(10):1071-80. doi: 10.1016/j.phytochem.2010.11.012. Epub 2010 Dec 15.
Mitochondrial NADH dehydrogenase complex (complex I) is by far the largest protein complex of the respiratory chain. It is best characterized for bovine mitochondria and known to consist of 45 different subunits in this species. Proteomic analyses recently allowed for the first time to systematically explore complex I from plants. The enzyme is especially large and includes numerous extra subunits. Upon subunit separation by various gel electrophoresis procedures and protein identifications by mass spectrometry, overall 47 distinct types of proteins were found to form part of Arabidopsis complex I. An additional subunit, ND4L, is present but could not be detected by the procedures employed due to its extreme biochemical properties. Seven of the 48 subunits occur in pairs of isoforms, six of which were experimentally proven. Fifteen subunits of complex I from Arabidopsis are specific for plants. Some of these resemble enzymes of known functions, e.g. carbonic anhydrases and l-galactono-1,4-lactone dehydrogenase (GLDH), which catalyzes the last step of ascorbate biosynthesis. This article aims to review proteomic data on the protein composition of complex I in plants. Furthermore, a proteomic re-evaluation on its protein constituents is presented.
线粒体 NADH 脱氢酶复合物（复合物 I）是迄今为止呼吸链中最大的蛋白质复合物。它在牛线粒体中表现得最为明显，已知在该物种中由 45 个不同的亚基组成。蛋白质组学分析最近首次允许系统地研究植物中的复合物 I。该酶特别大，包含许多额外的亚基。通过各种凝胶电泳程序分离亚基，并通过质谱法进行蛋白质鉴定，总共发现了 47 种不同类型的蛋白质形成拟南芥复合物 I 的一部分。另一个亚基 ND4L 存在，但由于其极端的生化特性，所采用的程序无法检测到。48 个亚基中的 7 个存在同工型对，其中 6 个已通过实验证明。拟南芥复合物 I 的 15 个亚基是植物特有的。其中一些类似于已知功能的酶，例如碳酸酐酶和 l-半乳糖酸-1,4-内酯脱氢酶（GLDH），它催化抗坏血酸生物合成的最后一步。本文旨在综述有关植物中复合物 I 蛋白质组成的蛋白质组学数据。此外，还对其蛋白质成分进行了蛋白质组学再评估。
