Li Chunji, Li Bingxue, Zhang Ning, Wei Na, Wang Qifan, Wang Wenjing, Xie Yiwei, Zou Hongtao
College of Land and Environment, Shenyang Agricultural University.
College of Bioscience and Biotechnology, Shenyang Agricultural University.
J Gen Appl Microbiol. 2019 Jul 19;65(3):111-120. doi: 10.2323/jgam.2018.07.001. Epub 2018 Nov 29.
Carotenoids represent a diverse class of aliphatic C40 molecules with a variety of applications in the food and pharmaceutical industries. Sporidiobolus pararoseus NGR produces various carotenoids, including torulene, torularhodin and β-carotene. Salt stress significantly increases the torulene accumulation of S. pararoseus NGR. However, little is known, about the molecular mechanisms underlying the increased torulene biosynthesis. In this work, we investigated the effects of NaCl treatment on the contents of carotenoids (both qualitatively and quantitatively) and transcriptome. A total of 12.3 Gb of clean bases were generated in six cDNA libraries. These bases were de novo assembled into 9,533 unigenes with an average length of 1,654 nt and N50 of 2,371 nt. Transcriptome analysis revealed that of 3,849 differential expressed genes (DEGs) in response to salt stress, 2,019 were up-regulated, and 1,830 were down-regulated. Among these DEGs, we identified three carotenogenic genes crtE, crtYB, and crtI. In addition, fourteen candidate genes were predicted to participate in the conversion from torulene to torularhodin. Our findings should provide insights into the mechanisms of carotenoid biosynthesis and salt-tolerance of S. pararoseus NGR.
类胡萝卜素是一类多样的脂肪族C40分子，在食品和制药行业有多种应用。嗜玫色掷孢酵母NGR能产生多种类胡萝卜素，包括圆酵母素、圆酵母红素和β-胡萝卜素。盐胁迫显著增加了嗜玫色掷孢酵母NGR中圆酵母素的积累。然而，关于圆酵母素生物合成增加的分子机制却知之甚少。在这项研究中，我们研究了NaCl处理对类胡萝卜素含量（包括定性和定量）以及转录组的影响。六个cDNA文库共产生了12.3 Gb的干净碱基。这些碱基被从头组装成9533个单基因，平均长度为1654 nt，N50为2371 nt。转录组分析显示，在响应盐胁迫的3849个差异表达基因（DEG）中，2019个上调，1830个下调。在这些DEG中，我们鉴定出三个类胡萝卜素生成基因crtE、crtYB和crtI。此外，预测有14个候选基因参与从圆酵母素到圆酵母红素的转化。我们的研究结果应为嗜玫色掷孢酵母NGR的类胡萝卜素生物合成和耐盐机制提供见解。
