College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.
College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.
Food Chem. 2017 Dec 15;237:1041-1047. doi: 10.1016/j.foodchem.2017.06.033. Epub 2017 Jun 8.
Carotenoids represent a class of molecules valuable for food, chemical and pharmaceutical industries. As a microbial carotenoid, torulene possesses potential health-promoting effects in human. However, few studies have been conducted to investigate optimal condition for its large-scale commercial production to date. Sporidiobolus pararoseus NGR, a pigmented yeast, was shown previously to accumulate considerable amounts of torulene, β-carotene, and torularhodin. In this study, the effect of salt stress on the production of carotenoids in S. pararoseus NGR was investigated. After 5days of cultivation, the total amount of carotenoids was significantly higher in 0.75M (3.952mg/L) and 1M (2.89mg/L) NaCl treatments than control (1.636mg/L), respectively. Among them, the increase in torulene accumulation is the main contributor to the improvement in total amount of carotenoids under 0.75 and 1M NaCl treatments. Together, our results should advance the development of metabolic engineering for the commercial production of torulene.
类胡萝卜素是一类对食品、化学和制药工业具有重要价值的分子。作为一种微生物类胡萝卜素，角黄素在人体中具有潜在的促进健康的作用。然而，迄今为止，很少有研究探讨其大规模商业化生产的最佳条件。先前的研究表明，产朊假丝酵母 NGR 是一种色素酵母，可以积累相当数量的角黄素、β-胡萝卜素和 torularhodin。在这项研究中，研究了盐胁迫对产朊假丝酵母 NGR 中产类胡萝卜素的影响。培养 5 天后，在 0.75M（3.952mg/L）和 1M（2.89mg/L）NaCl 处理组中的类胡萝卜素总量明显高于对照组（1.636mg/L）。其中，角黄素积累的增加是导致 0.75 和 1M NaCl 处理组中类胡萝卜素总量增加的主要原因。总之，我们的研究结果将有助于推进商业生产角黄素的代谢工程的发展。
