The synergy of dietary supplements LI01 and TC01 in alleviating liver failure in rats treated with D-galactosamine.

() has been widely used in dietary supplements and clinical treatments. Previous studies demonstrated the protective effect of LI01 on liver injury induced by D-galactosamine (D-GaIN) in rats. Accumulating evidence indicates that and are highly coordinated; so in this study, we focus on the synergistic effect of LI01 and TC01 on the alleviation of liver injury caused by D-GaIN in rats and aim to find out the underlying interaction between the two strains. We observed reduced hepatic damage in the D-GaIN-treated rats with probiotic pre-administration, characterized by lower levels of AST and ALT ( < 0.05) and decreased HAI (Histological Activity Index) scores. Moreover, cotreatment with LI01 and TC01 more effectively decreases proinflammatory cytokines TNF-α, MCP-1 and M-CSF ( < 0.05) so as to inhibit systemic inflammation. Gut barrier dysfunction was ameliorated with compound probiotic pretreatment, as evidenced by the ultrastructure integrity, decreased histological score and elevated TJP-1 expression. What's more, supplementation with LI01 and TC01 markedly alleviates gut dysbiosis in the G-DaIN-treated rats, with enrichment of short chain fatty acid (SCFA) producers and group, a decreased / (F/B) ratio and depletion of proinflammatory microbes, such as and . This study highlights the synergistic effect of dietary supplements LI01 and TC01 on the protection against liver failure, which is probably altering gut microbiota.