Department of Plant Pathology and Microbiology, Texas A&M AgriLife Research and Extension Center, Weslaco, TX, 78596, USA.
Department of Plant Pathology, University of California-Davis, Davis, CA, 95616, USA.
Arch Virol. 2021 Dec;166(12):3399-3404. doi: 10.1007/s00705-021-05218-4. Epub 2021 Sep 21.
Olea europaea geminivirus (OEGV) from olive accessions in Italy was characterized recently. OEGV was also detected during routine high-throughput sequencing screening of olive (cv. Leccino) material, and its complete bipartite genome segments were sequenced and shown to be 100% identical to those of the isolate from Italy. Using two pairs of newly designed primers targeting the AV1 and BV1 genes, OEGV was detected in randomly sampled olive trees from the U.S. Department of Agriculture National Clonal Germplasm Repository (USDA-NCGR) (21.4% or 6/28), commercial and residential settings in California (47.6% or 10/21), and an orchard in Texas (60% or 30/50). The cuttings for the USDA-NCGR-positive trees originated from the former Serbia and Montenegro, Spain, Italy, and Greece. Comparative analysis of the directly sequenced gene fragments from randomly selected samples showed that OEGV isolates from the different sources were 100% identical to each other. The results indicate that OEGV spread was likely facilitated by inadvertent movement of contaminated olive germplasm.
最近，对来自意大利橄榄属植物的麻疯树双生病毒（OEGV）进行了特征描述。在对橄榄（Leccino 品种）材料进行常规高通量测序筛选的过程中，也检测到了 OEGV，其完整的双份基因组片段被测序，并显示与来自意大利的分离物完全相同。使用两对针对 AV1 和 BV1 基因设计的新引物，在美国农业部国家无性系种质资源库（USDA-NCGR）中随机采样的橄榄树中检测到了 OEGV（21.4%或 6/28），加利福尼亚州的商业和住宅环境中（47.6%或 10/21），以及德克萨斯州的一个果园（60%或 30/50）。USDA-NCGR 阳性树的插条来自前塞尔维亚和黑山、西班牙、意大利和希腊。对随机选择的样本进行直接测序的基因片段的比较分析表明，来自不同来源的 OEGV 分离物彼此完全相同。结果表明，OEGV 的传播可能是由于受污染的橄榄种质的无意转移而促成的。
