The Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, College of Animal Science, Yangtze University, Jingzhou, China.
College of Veterinary Medicine, Shandong Agricultural University, Taìan, China.
Anim Biotechnol. 2023 Jun;34(3):508-519. doi: 10.1080/10495398.2021.1979022. Epub 2021 Sep 22.
This study aimed to evaluate the immune effects of compound astragalus polysaccharide and sulfated epimedium polysaccharide (APS-sEPS) on the peripheral blood lymphocyte and intestinal mucosa in newborn piglets. A total of 40 newborn piglets were randomly divided into four groups during a 25-day experiment, including APS-sEPS, APS, sEPS and control group. The results showed that supplementation with APS-sEPS to newborn piglets remarkably increased the physiological parameters, especially the WBC. In peripheral blood, piglets that received APS-sEPS showed the highest proliferation of T lymphocytes, the percentage of CD3 + CD4+ and CD3 + CD8+ cells were the highest on days 15 and 25 ( < 0.05). The serum concentrations of IFN-γ on days 7 and 15, and IL-4, IL-10, sIgA on days 7, 15 and 25 in APS-sEPS group were significantly higher than those in the control group ( < 0.05). Furthermore, the villus length and the ratio of villus length to crypt depth in APS-sEPS group were both significantly increased compared to that of control group ( < 0.05). In the duodenum, jejunum and illume, the concentrations of IFN-γ, IL-10, total IgG and sIgA in APS-sEPS group were all significantly higher than that in control group ( < 0.05). In intestinal mucosa, APS-sEPS significantly increased the expression of and mRNA in each section of small intestine of piglets. Nevertheless, in the illume segment, the effect of APS-sEPS was more significant than that of APS and sEPS ( < 0.05). The expression of was more significant than that of control group in duodenum only. The results from the present research provide evidence that the suckling piglets administered with APS-sEPS supplement exhibited enhanced immune function of peripheral blood lymphocyte and expression of specific antibodies, and ameliorated intestinal morphological development and increased activities of humoral immune response in the small intestine, which would be related to the activation of the signaling pathway and .
本研究旨在评估复方黄芪多糖和硫酸淫羊藿多糖（APS-sEPS）对新生仔猪外周血淋巴细胞和肠黏膜的免疫作用。在为期 25 天的实验中，将 40 头新生仔猪随机分为四组，包括 APS-sEPS、APS、sEPS 和对照组。结果表明，给新生仔猪补充 APS-sEPS 可显著提高生理参数，尤其是白细胞数。在外周血中，接受 APS-sEPS 处理的仔猪在第 15 天和第 25 天的 T 淋巴细胞增殖最高，CD3+CD4+和 CD3+CD8+细胞的百分比最高（<0.05）。第 7 天和第 15 天 APS-sEPS 组血清 IFN-γ浓度，第 7、15 和 25 天 APS-sEPS 组血清 IL-4、IL-10、sIgA 浓度均显著高于对照组（<0.05）。此外，APS-sEPS 组的绒毛长度和绒毛长度与隐窝深度的比值均显著高于对照组（<0.05）。在十二指肠、空肠和回肠中，APS-sEPS 组 IFN-γ、IL-10、总 IgG 和 sIgA 浓度均显著高于对照组（<0.05）。在肠黏膜中，APS-sEPS 显著增加了仔猪各肠段 和 基因的表达。然而，在回肠段，APS-sEPS 的作用比 APS 和 sEPS 更显著（<0.05）。在十二指肠中，只有 的表达比对照组更显著。本研究结果表明，给哺乳仔猪补充 APS-sEPS 可增强外周血淋巴细胞的免疫功能和特异性抗体的表达，改善小肠的形态发育，增加小肠的体液免疫反应活性，这可能与 信号通路和 的激活有关。
