Zhao Yan, Chen Haitao, Zhang Wenhui, Shang Wei, Cao Jinwei, Zhao Huijuan, Zou Zhixiang
Department of Gynecology, Taiyuan Central Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi, China.
Department of Obstetrics and Gynecology, Zhucheng Hospital of Traditional Chinese Medicine, Weifang, 262200, Shandong, China.
Hereditas. 2025 Jan 9;162(1):4. doi: 10.1186/s41065-024-00363-7.
Cervical cancer (CC) is a prevalent gynecological malignancy, contributing to a substantial number of fatalities among women. MicroRNAs (miRNAs) have emerged as promising biomarkers with significant potential for the early detection and prognosis of CC.
This study aimed to explore the clinical significance and biological role of miR-615-5p in CC, with the goal of identifying novel biomarkers for this disease.
The levels of miR-615-5p and TMIGD2 mRNA in tissue samples and cells were quantified through quantitative reverse transcription real-time PCR, followed by statistical analyses to investigate the correlation between miR-615-5p and clinical data. The effects of miR-615-5p on the proliferation and metastasis of CC cells were evaluated using the Cell Counting Kit-8 and Transwell assays. The potential mechanism of miR-615-5p was elucidated by bioinformatics analyses and Dual-luciferase reporter assay. Western blotting was employed to measure the protein levels of TMIGD2.
In CC, the downregulation of miR-615-5p was related to poor prognosis and emerged as an independent prognostic factor. The levels of miR-615-5p were reduced in CC cells. miR-615-5p overexpression restrained the proliferation and metastasis of CC cells. Furthermore, TMIGD2 was identified as a target gene regulated by miR-615-5p, and its expression was notably elevated in CC. The influence of miR-615-5p on the biological behaviors of CC cells was mediated through the modulation of TMIGD2.
Downregulation of miR-615-5p was a prognostic indicator of poor prognosis in CC. miR-615-5p exerted its tumor-suppressive effects by inhibiting cell growth and metastasis through the regulation of TMIGD2.
宫颈癌（CC）是一种常见的妇科恶性肿瘤，导致大量女性死亡。微小RNA（miRNA）已成为有前景的生物标志物，在CC的早期检测和预后方面具有巨大潜力。
本研究旨在探讨miR-615-5p在CC中的临床意义和生物学作用，以寻找该疾病的新型生物标志物。
通过定量逆转录实时PCR对组织样本和细胞中miR-615-5p和TMIGD2 mRNA的水平进行定量，随后进行统计分析以研究miR-615-5p与临床数据之间的相关性。使用细胞计数试剂盒-8和Transwell实验评估miR-615-5p对CC细胞增殖和转移的影响。通过生物信息学分析和双荧光素酶报告基因实验阐明miR-615-5p的潜在机制。采用蛋白质免疫印迹法检测TMIGD2的蛋白水平。
在CC中，miR-615-5p的下调与预后不良相关，并成为独立的预后因素。CC细胞中miR-615-5p的水平降低。miR-615-5p过表达抑制了CC细胞的增殖和转移。此外，TMIGD2被鉴定为受miR-615-5p调控的靶基因，其在CC中的表达显著升高。miR-615-5p对CC细胞生物学行为的影响是通过调节TMIGD2介导的。
miR-615-5p的下调是CC预后不良的一个预后指标。miR-615-5p通过调控TMIGD2抑制细胞生长和转移，发挥其肿瘤抑制作用。
