On Stephen L W, Zhang Junwen, Cornelius Angela J, Anderson Trevor P
Wine, Food and Molecular Biosciences, Lincoln University, PO Box 85084, Lincoln, New Zealand.
Institute of Environmental Science, Christchurch Science Centre, Christchurch, New Zealand.
Curr Res Microb Sci. 2020 Dec 16;2:100019. doi: 10.1016/j.crmicr.2020.100019. eCollection 2021 Dec.
Strains identified as may belong to one of at least two biochemically indistinguishable, but genomically distinct, groups referred to as "genomospecies" that may differ in their pathogenic and zoonotic potential. Reliable, affordable and available identification methods are required to improve understanding of their significance in human illness. We examined the potential for MALDI-TOF MS, increasingly used in routine laboratories, for this task. Nineteen well-characterised strains were examined using a widely used MALDI-TOF MS commercial system, however only one strain confidently identified using their database. Data mining of the spectra obtained revealed a number of markers that could be used to help discriminate these genomospecies. We conclude that careful application of MALDI-TOF analysis could be useful to determine the role and significance of diverse genomospecies in human disease.
被鉴定为[具体菌株名称未给出]的菌株可能至少属于两个生物化学特征无法区分但基因组不同的组之一，这两个组被称为“基因组种”，它们在致病和人畜共患病潜力方面可能存在差异。需要可靠、经济且可用的鉴定方法来增进对它们在人类疾病中重要性的理解。我们研究了在常规实验室中越来越常用的基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）用于此项任务的潜力。使用一种广泛使用的MALDI-TOF MS商业系统对19株特征明确的菌株进行了检测，然而使用其数据库仅能可靠鉴定出一株菌株。对所获得光谱的数据挖掘揭示了一些可用于帮助区分这些基因组种的标志物。我们得出结论，谨慎应用MALDI-TOF分析可能有助于确定不同基因组种在人类疾病中的作用和重要性。
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