评价 mRNA-1273 疫苗接种的医护人员中使用的 QuantiFERON SARS-CoV-2 干扰素-ɣ 释放检测。
Evaluation of the QuantiFERON SARS-CoV-2 interferon-ɣ release assay in mRNA-1273 vaccinated health care workers.
机构信息
Laboratory Diagnostic Center, University Hospital RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany.
Laboratory Diagnostic Center, University Hospital RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany.
出版信息
J Virol Methods. 2021 Dec;298:114295. doi: 10.1016/j.jviromet.2021.114295. Epub 2021 Sep 20.
Current studies focus on cellular and humoral immunity induced by novel SARS-CoV-2 vaccines. Non-responders to vaccinations are not uncommonly encountered in clinical medicine (e.g. in the field of hepatitis B). Whereas vaccine-induced humoral immunity against SARS-CoV-2 is compromised by emerging Variants of Concern (VOCs), cellular immunity against SARS-CoV-2 is emerging as resilient against VOCs. Thus commercially available test kits for diagnostic laboratories designed to evaluate cellular immune responses to SARS-CoV-2 are urgently needed. Here we evaluated the novel QuantiFERON SARS-CoV-2 assay (Qiagen) measuring INF-ɣ release induced by two spike-derived peptide pools (Ag1 and Ag2) in a cohort of health care workers vaccinated with the mRNA-1273 vaccine and confirmed humoral response. Our study indicates the usefulness of this novel assay for routine laboratories to evaluate cellular immunity against SARS-CoV-2 in response to mRNA-1273 vaccination.
目前的研究集中在新型 SARS-CoV-2 疫苗引起的细胞和体液免疫上。在临床医学中，（例如在乙肝领域）经常会遇到对疫苗无反应者。虽然针对 SARS-CoV-2 的疫苗诱导的体液免疫受到新出现的关注变体（VOCs）的影响，但针对 SARS-CoV-2 的细胞免疫似乎对 VOCs 具有弹性。因此，迫切需要用于诊断实验室的商用试剂盒来评估针对 SARS-CoV-2 的细胞免疫反应。在这里，我们评估了新型 QuantiFERON SARS-CoV-2 测定法（Qiagen），该测定法测量了 mRNA-1273 疫苗接种的医护人员中由两个刺突衍生肽池（Ag1 和 Ag2）诱导的 INF-ɣ 释放，并确认了体液反应。我们的研究表明，该新型测定法对于常规实验室在针对 mRNA-1273 疫苗接种的 SARS-CoV-2 细胞免疫评估方面非常有用。
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