Zucconi G G, Belia S, Menichini E, Castigli E, Giuditta A
Institute of Cell Biology, Perugia, Italy.
Int J Dev Neurosci. 1986;4(2):169-78. doi: 10.1016/0736-5748(86)90042-0.
Pregnant rats were deprived of paradoxical sleep for 3 days starting on the 18th gestational day. The condition of PS-D was imposed by confinement on a small platform surrounded by water or by daily injections of clomipramine. Four hours before the killing rats received a s.c. injection of [3H]-thymidine. The amount of radioactive DNA determined by autoradiography in several regions of fetal brain was found to be markedly increased under both experimental conditions in comparison with the control fetal brain. Considerably more limited effects were observed in kidney. Comparable changes of lower magnitude were obtained by comparing the specific radioactivity of DNA samples purified by chlorophorm extraction and digestion with RNase and proteinase K. The results fully confirm our previous data obtained under similar experimental conditions but based on the analysis of an acid-washed DNA fraction.
妊娠大鼠从妊娠第18天开始被剥夺异相睡眠3天。通过将其限制在被水包围的小平台上或每日注射氯米帕明来造成异相睡眠剥夺（PS-D）状态。在处死大鼠前4小时，给它们皮下注射[3H] - 胸腺嘧啶核苷。通过放射自显影法测定发现，与对照胎儿脑相比，在两种实验条件下，胎儿脑几个区域中放射性DNA的量均显著增加。在肾脏中观察到的影响则明显更有限。通过比较用氯仿提取、用核糖核酸酶和蛋白酶K消化后纯化的DNA样品的比放射性，获得了程度较低的类似变化。这些结果充分证实了我们先前在类似实验条件下基于酸洗DNA组分分析所获得的数据。
