Wetzel W, Schmidt U, Wagner T, Matthies H
Institute of Neurobiology and Brain Research, Academy of Sciences, Magdeburg, GDR.
Brain Res Bull. 1990 Jun;24(6):743-6. doi: 10.1016/0361-9230(90)90133-k.
Brain extract was obtained from whole brains of donor rats following four days of paradoxical sleep deprivation (PSD). Intraperitoneal injection of this brain extract in normal (i.e., not pretreated) recipient rats led to a significant increase of paradoxical sleep (PS) which was beginning at the third hour postinjection and still present on the day after treatment. Both PS episode number and PS episode duration were increased by the PSD extract, whereas slow-wave sleep was not influenced. Brain extract of nondeprived control rats, on the other hand, had no significant effects. In the present experiments, an extract volume obtained from two donor brains was transferred to one recipient. Compared to our earlier investigations (one donor brain/one recipient) this higher "dose" of PSD extract exerted a more pronounced and prolonged effect. These results support the idea that some PS-inducing factor(s) accumulating in the brain during PS deprivation may be involved in the regulation of paradoxical sleep.
在进行四天的异相睡眠剥夺（PSD）后，从供体大鼠的全脑中获取脑提取物。将这种脑提取物腹腔注射到正常（即未预处理）的受体大鼠中，会导致异相睡眠（PS）显著增加，这种增加在注射后第三小时开始，并在治疗后的第二天仍然存在。PSD提取物增加了PS发作次数和PS发作持续时间，而慢波睡眠则未受影响。另一方面，未剥夺的对照大鼠的脑提取物没有显著影响。在本实验中，将从两个供体脑获得的提取物体积转移到一个受体中。与我们早期的研究（一个供体脑/一个受体）相比，这种更高“剂量”的PSD提取物产生了更明显和持久的效果。这些结果支持这样一种观点，即在PS剥夺期间大脑中积累的一些PS诱导因子可能参与异相睡眠的调节。
