College of Chemistry and Molecular Sciences, Hubei Engineering Center of Natural Polymer-based Medical Materials, Wuhan University, Wuhan 430072, China.
Engineering Research Center for Biomedical Materials, Anhui Key Laboratory of Modern Biomanufacturing, School of Life Sciences, Anhui University, 111 Jiulong Road, Hefei, Anhui Province 230601, China.
Carbohydr Polym. 2021 Dec 1;273:118547. doi: 10.1016/j.carbpol.2021.118547. Epub 2021 Aug 11.
To meet the rising demand of injectable hydrogels with self-healing, robustness and biocompatibility for biomedical engineering, the reversible ketoester-type acylhydrazone linkages was used for the fabrication of novel cellulose-based hydrogel. The ketoester-type acylhydrazone bond exchanged rapidly, endowing the hydrogels with highly efficient self-healing performance without any external stimuli under physiological environment, which was hardly achieved with the widely used arylhydrozone bond. The dynamic hydrogels exhibited tunable mechanical property, pH responsiveness, injectability and biocompatibility, demonstrating immense applications prospect for various biomedicines, such as drug and cell delivery. The pH-responsive controlled release of model drug doxorubicin (DOX) loaded in the hydrogel was demonstrated. In addition, benefitting from the excellent biocompatibility and the reversible ketoester-type acylhydrazone bonds, cells were encapsulated in the hydrogels as 3D carrier. The covalent adaptable network intensified injectability of cell-laden hydrogels and improved the long-lasting viability for cell culture, showing great potential in the biomedical field.
为满足生物医学工程领域对具有自修复、强韧和生物相容性的可注射水凝胶不断增长的需求，利用可快速交换的酮酯型酰腙键来制备新型纤维素基水凝胶。与广泛应用的芳基腙键相比，酮酯型酰腙键无需外部刺激即可在生理环境下赋予水凝胶高效的自修复性能，这是很难实现的。动态水凝胶表现出可调的机械性能、pH 响应性、可注射性和生物相容性，为各种生物医学应用，如药物和细胞输送，展示了广阔的应用前景。研究表明，载药水凝胶中模型药物阿霉素（DOX）具有 pH 响应性控制释放性能。此外，得益于优异的生物相容性和可交换的酮酯型酰腙键，细胞被封装在水凝胶中作为 3D 载体。共价适应性网络增强了载细胞水凝胶的可注射性，并提高了细胞培养的长期存活率，在生物医学领域具有巨大的应用潜力。
