Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong.
Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong.
Carbohydr Polym. 2020 May 15;236:116074. doi: 10.1016/j.carbpol.2020.116074. Epub 2020 Feb 26.
Pluronic F-127 based dual-responsive (pH/temperature) hydrogel drug delivery system was developed involving polysaccharide-based nano-conjugate of hyaluronic acid and chitosan oligosaccharide lactate and applied for loading of gallic acid which is the principal component of traditional Chinese medicine Cortex Moutan recommended in the treatment of atopic dermatitis. The polysaccharide-based nano-conjugate was used as pH-responsive compound in the formulation and its amphiphilic character was determined colorimetrically. Microstructure analysis by SEM and TEM indicated highly porous hydrogel network and well-dispersed micellar structures, respectively, after modification with the nano-conjugate, and so, release property of the hydrogel for drug was significantly improved. Different pH-conditions were applied here to see pH-responsiveness of the formulation and increase in acidity of external environment gradually diminished mechanical stability of the hydrogel and that was reflected on the drug release property. Rheology was performed to observe sol-gel transition of the formulation and showed better rheological properties after modification with nano-conjugate. In this study, the cytotoxicity results of PF127 based formulations loaded with/without gallic acid showed cell viability of > 80.0 % for human HaCaT keratinocytes in the concentration range of 0.0-20.0 μg/ml.
基于 Pluronic F-127 的双响应（pH/温度）水凝胶药物递送系统的开发涉及基于多糖的透明质酸和壳聚糖寡糖乳酸的纳米缀合物，并应用于加载没食子酸，没食子酸是传统中药牡丹皮中治疗特应性皮炎的主要成分。基于多糖的纳米缀合物被用作配方中的 pH 响应化合物，并通过比色法确定其两亲性。SEM 和 TEM 的微观结构分析表明，经过纳米缀合物修饰后，水凝胶具有高度多孔的网络结构和良好分散的胶束结构，因此，水凝胶对药物的释放性能得到显著改善。这里应用了不同的 pH 条件来观察配方的 pH 响应性，外部环境酸度的增加逐渐降低了水凝胶的机械稳定性，这反映在药物释放特性上。流变学用于观察配方的溶胶-凝胶转变，并显示出经过纳米缀合物修饰后的更好的流变性能。在这项研究中，载有/不载有没食子酸的 PF127 制剂的细胞毒性结果表明，在浓度范围为 0.0-20.0μg/ml 时，人 HaCaT 角质形成细胞的细胞活力>80.0%。
