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嗜盐菌 sp. EG9S8QL 中 4-O-[1-羧乙基]-d-甘露糖的结构。含有的 O-特异性多糖。

Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium sp. EG9S8QL.

机构信息

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia.

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky Prospekt, 119991 Moscow, Russia.

出版信息

Mar Drugs. 2021 Sep 7;19(9):508. doi: 10.3390/md19090508.

DOI:10.3390/md19090508
PMID:34564170
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8466920/
Abstract

The moderately halophilic strain sp. EG9S8QL was isolated among 11 halophilic strains from saline mud (Emisal Salt Company, Lake Qarun, Fayoum, Egypt). The lipopolysaccharide was extracted from dried cells of sp. EG9S8QL by the phenol-water procedure. The OPS was obtained by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with H and C NMR spectroscopy, including H,H COSY, TOCSY, ROESY, H,C HSQC, and HMBC experiments. The OPS was found to be composed of linear tetrasaccharide repeating units of the following structure: →2)-β-Man4Lac-(1→3)-α-ManNAc-(1→3)-β-Rha-(1→4)-α-GlcNAc-(1→, where Man4Lac is 4-O-[1-carboxyethyl]mannose.

摘要

中度嗜盐菌株 sp. EG9S8QL 是从埃及 Fayoum 地区的卡伦湖盐泥中分离出来的 11 株嗜盐菌株之一。从 sp. EG9S8QL 的干燥细胞中用苯酚-水法提取脂多糖。OPS 通过脂多糖的温和酸水解得到，并通过糖分析以及 H 和 C NMR 光谱学研究，包括 H,H COSY、TOCSY、ROESY、H,C HSQC 和 HMBC 实验进行研究。OPS 由以下结构的线性四糖重复单元组成：→2）-β-Man4Lac-（1→3）-α-ManNAc-（1→3）-β-Rha-（1→4）-α-GlcNAc-（1→，其中 Man4Lac 是 4-O-[1-羧乙基]甘露糖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/cab153fa409a/marinedrugs-19-00508-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/ab6be81ec762/marinedrugs-19-00508-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/f5b5fe0bd80e/marinedrugs-19-00508-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/02905ff78b2c/marinedrugs-19-00508-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/47eed088db2d/marinedrugs-19-00508-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/368eb6fd247d/marinedrugs-19-00508-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/fff79733d783/marinedrugs-19-00508-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/cab153fa409a/marinedrugs-19-00508-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/ab6be81ec762/marinedrugs-19-00508-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/f5b5fe0bd80e/marinedrugs-19-00508-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/02905ff78b2c/marinedrugs-19-00508-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/47eed088db2d/marinedrugs-19-00508-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/368eb6fd247d/marinedrugs-19-00508-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/fff79733d783/marinedrugs-19-00508-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d583/8466920/cab153fa409a/marinedrugs-19-00508-g007.jpg

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