Rowan Daryl, Boldingh Helen, Cordiner Sarah, Cooney Janine, Hedderley Duncan, Hewitt Katrin, Jensen Dwayne, Pereira Trisha, Trower Tania, McGhie Tony
Fitzherbert Science Centre, The New Zealand Institute for Plant and Food Research Limited, Batchelar Road, Palmerston North 4410, New Zealand.
Ruakura Research Centre, The New Zealand Institute for Plant and Food Research Limited, Bisley Road, Hamilton 3214, New Zealand.
Metabolites. 2021 Sep 6;11(9):603. doi: 10.3390/metabo11090603.
Plant metabolomics within field-based food production systems is challenging owing to environmental variability and the complex architecture and metabolic growth cycles of plants. Kiwifruit cultivars of are vigorous perennial vines grown as clones in highly structured orchard environments, intensively managed to maximize fruit yield and quality. To understand the metabolic responses of vines to orchard management practices, we needed to better understand the various sources of metabolic variability encountered in the orchard. Triplicate composite leaf, internode and fruit (mature and immature) samples were collected from each of six var. 'Hayward' and var. 'Zesy002' kiwifruit vines at three times during the growing season and measured by LC-MS. In general, there was more variation in metabolite concentrations within vines than between vines, with 'Hayward' showing a greater percentage of within-vine variability than 'Zesy002' (c. 90 vs. 70% respectively). In specific tissues, the sampler, infection by var. and the rootstock also influenced metabolite variability. A similar pattern of metabolic variability was observed from quantitative analysis of specific carbohydrates and phytohormones. High within-vine metabolic variability indicates that it is more important to obtain sufficient replicate samples than to sample from multiple vines. These data provide an objective basis for optimizing metabolite sampling strategies within kiwifruit orchards.
由于环境的变异性以及植物复杂的结构和代谢生长周期，基于田间的粮食生产系统中的植物代谢组学研究具有挑战性。奇异果品种是多年生藤本植物，在高度结构化的果园环境中作为克隆植株种植，通过集约化管理以实现果实产量和品质的最大化。为了了解藤蔓对果园管理措施的代谢反应，我们需要更好地理解果园中遇到的各种代谢变异性来源。在生长季节的三个时间点，从六个‘海沃德’品种和‘Zesy002’品种的奇异果藤蔓中分别采集了三份复叶、节间和果实（成熟和未成熟）样本，并通过液相色谱-质谱联用仪进行测量。总体而言，藤蔓内部代谢物浓度的变化比藤蔓之间的变化更大，‘海沃德’品种藤蔓内部变异性的百分比高于‘Zesy002’品种（分别约为90%和70%）。在特定组织中，采样器、被某变种感染以及砧木也会影响代谢物的变异性。从特定碳水化合物和植物激素的定量分析中也观察到了类似的代谢变异性模式。藤蔓内部较高的代谢变异性表明，获取足够的重复样本比从多个藤蔓上采样更为重要。这些数据为优化奇异果果园内的代谢物采样策略提供了客观依据。
