• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基因座激活区域的证据：在表达珠蛋白的杂种中形成发育稳定的超敏位点。

Evidence for a locus activation region: the formation of developmentally stable hypersensitive sites in globin-expressing hybrids.

作者信息

Forrester W C, Takegawa S, Papayannopoulou T, Stamatoyannopoulos G, Groudine M

机构信息

Division of Basic Sciences, Genetics, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

出版信息

Nucleic Acids Res. 1987 Dec 23;15(24):10159-77. doi: 10.1093/nar/15.24.10159.

DOI:10.1093/nar/15.24.10159
PMID:3480506
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339937/
Abstract

We have analyzed the chromatin structure of the human beta-globin locus in somatic cell hybrids resulting from the fusion of human non-erythroid cells and mouse erythroleukemia (MEL) cells. In these hybrids, the human adult beta-globin gene, but neither the embryonic nor fetal globin genes, is activated transcriptionally. In addition, the DNase I-resistant beta-like globin locus characteristic of the parental non-erythroid human cells (1,2) is reorganized over an approximately 80 kb region, including the formation of the developmentally stable hypersensitive sites 50 kb 5' and 20 kg 3' of the activated adult beta-globin gene (2,3). These results are consistent with the hypothesis that events occurring at the 5' and/or 3' developmentally stable hypersensitive sites are important, if not necessary, for the activation of the beta-globin locus.

摘要

我们分析了人非红细胞与小鼠红白血病（MEL）细胞融合产生的体细胞杂种中人类β-珠蛋白基因座的染色质结构。在这些杂种中，人类成人β-珠蛋白基因被转录激活，但胚胎和胎儿珠蛋白基因均未被激活。此外，亲本非红细胞人类细胞特有的对DNA酶I有抗性的β-样珠蛋白基因座在大约80 kb的区域内发生了重组，包括在激活的成人β-珠蛋白基因5'端50 kb和3'端20 kb处形成发育稳定的超敏感位点（2,3）。这些结果与以下假设一致，即发生在5'和/或3'发育稳定超敏感位点的事件对于β-珠蛋白基因座的激活很重要，即使不是必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/2a0de9bc2d9d/nar00268-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/79762ef2dd3d/nar00268-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/f1bfb222d16a/nar00268-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/957b6e846ebb/nar00268-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/1804085f75d5/nar00268-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/2e9bd145771c/nar00268-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/2a0de9bc2d9d/nar00268-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/79762ef2dd3d/nar00268-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/f1bfb222d16a/nar00268-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/957b6e846ebb/nar00268-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/1804085f75d5/nar00268-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/2e9bd145771c/nar00268-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d64/339937/2a0de9bc2d9d/nar00268-0100-a.jpg

相似文献

1
Evidence for a locus activation region: the formation of developmentally stable hypersensitive sites in globin-expressing hybrids.基因座激活区域的证据：在表达珠蛋白的杂种中形成发育稳定的超敏位点。
Nucleic Acids Res. 1987 Dec 23;15(24):10159-77. doi: 10.1093/nar/15.24.10159.
2
Mechanism of DNase I hypersensitive site formation within the human globin locus control region.人类珠蛋白基因座控制区内DNase I超敏位点形成的机制。
Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):1143-7. doi: 10.1073/pnas.89.3.1143.
3
A deletion of the human beta-globin locus activation region causes a major alteration in chromatin structure and replication across the entire beta-globin locus.人类β-珠蛋白基因座激活区域的缺失会导致整个β-珠蛋白基因座的染色质结构和复制发生重大改变。
Genes Dev. 1990 Oct;4(10):1637-49. doi: 10.1101/gad.4.10.1637.
4
High-level beta-globin expression after retroviral transfer of locus activation region-containing human beta-globin gene derivatives into murine erythroleukemia cells.将含基因座激活区域的人β-珠蛋白基因衍生物通过逆转录病毒转移至小鼠红白血病细胞后，β-珠蛋白的高水平表达。
Proc Natl Acad Sci U S A. 1990 May;87(9):3386-90. doi: 10.1073/pnas.87.9.3386.
5
An erythroid-specific chromatin opening element reorganizes beta-globin promoter chromatin structure and augments gene expression.一个红系特异性染色质开放元件重组β-珠蛋白启动子染色质结构并增强基因表达。
Blood Cells Mol Dis. 2001 Jul-Aug;27(4):767-80. doi: 10.1006/bcmd.2001.0448.
6
Activation and repression of a beta-globin gene in cell hybrids is accompanied by a shift in its temporal replication.细胞杂交体中β-珠蛋白基因的激活和抑制伴随着其时间复制的改变。
Mol Cell Biol. 1989 Aug;9(8):3524-32. doi: 10.1128/mcb.9.8.3524-3532.1989.
7
A developmentally stable chromatin structure in the human beta-globin gene cluster.人类β-珠蛋白基因簇中一种发育稳定的染色质结构。
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1359-63. doi: 10.1073/pnas.83.5.1359.
8
Erythroid-specific nuclease-hypersensitive sites flanking the human beta-globin domain.人类β-珠蛋白结构域两侧的红系特异性核酸酶超敏位点。
Mol Cell Biol. 1990 Aug;10(8):4324-33. doi: 10.1128/mcb.10.8.4324-4333.1990.
9
The "beta-like-globin" gene domain in human erythroid cells.人类红细胞中的“类β-珠蛋白”基因结构域。
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6384-8. doi: 10.1073/pnas.82.19.6384.
10
Partial repression of human gamma-globin genes by LCR element HS3 when linked to beta-globin genes and LCR element HS2 in MEL cells.当与β-珠蛋白基因以及MEL细胞中的LCR元件HS2相连时，LCR元件HS3对人γ-珠蛋白基因的部分抑制作用。
Am J Hematol. 1996 Mar;51(3):220-8. doi: 10.1002/(SICI)1096-8652(199603)51:3<220::AID-AJH7>3.0.CO;2-Z.

引用本文的文献

1
Epigenetic Regulation of Erythropoiesis: From Developmental Programs to Therapeutic Targets.红细胞生成的表观遗传调控：从发育程序到治疗靶点
Int J Mol Sci. 2025 Jun 30;26(13):6342. doi: 10.3390/ijms26136342.
2
Current and future treatments for sickle cell disease: From hematopoietic stem cell transplantation to in vivo gene therapy.镰状细胞病的当前及未来治疗方法：从造血干细胞移植到体内基因治疗。
Mol Ther. 2025 May 7;33(5):2172-2191. doi: 10.1016/j.ymthe.2025.03.016. Epub 2025 Mar 12.
3
Ancestry of the major long-range regulatory site of the α-globin genes in the Portuguese population with the common 3.7 kb α-thalassemia deletion.

本文引用的文献

1
Selective in vitro transcription of one of the two Alu family repeats present in the 5' flanking region of the human epsilon-globin gene.对人类ε-珠蛋白基因5'侧翼区域存在的两个Alu家族重复序列之一进行选择性体外转录。
Nucleic Acids Res. 1981 Dec 21;9(24):6709-22. doi: 10.1093/nar/9.24.6709.
2
Human myeloid leukemia cell lines: a review.人类髓系白血病细胞系：综述。
Blood. 1980 Sep;56(3):344-50.
3
Human globin psi B2 is not a globin-related sequence.人类珠蛋白ψ B2不是与珠蛋白相关的序列。
葡萄牙人群中常见的 3.7kbα-地中海贫血缺失的α-珠蛋白基因主要长距离调控区的起源。
Mol Biol Rep. 2024 May 5;51(1):612. doi: 10.1007/s11033-024-09530-5.
4
Gene Therapy for β-Hemoglobinopathies: From Discovery to Clinical Trials.β-地中海贫血症的基因治疗：从发现到临床试验。
Viruses. 2023 Mar 9;15(3):713. doi: 10.3390/v15030713.
5
Genetic cold cases: lessons from solving complex congenital limb disorders.遗传冷案：从解决复杂先天性肢体畸形中获得的经验教训。
Genes Dev. 2023 Apr 1;37(7-8):261-276. doi: 10.1101/gad.350450.123. Epub 2023 Mar 29.
6
Molecular Basis and Genetic Modifiers of Thalassemia.地中海贫血的分子基础和遗传修饰物。
Hematol Oncol Clin North Am. 2023 Apr;37(2):273-299. doi: 10.1016/j.hoc.2022.12.001.
7
Coming full circle: On the origin and evolution of the looping model for enhancer-promoter communication.兜兜转转回到原点：增强子-启动子通讯的环出模型的起源与演化。
J Biol Chem. 2022 Aug;298(8):102117. doi: 10.1016/j.jbc.2022.102117. Epub 2022 Jun 9.
8
Super-enhancer mediated regulation of adult β-globin gene expression: the role of eRNA and Integrator.超级增强子介导的成人β-珠蛋白基因表达调控：eRNA 和 Integrator 的作用。
Nucleic Acids Res. 2021 Feb 22;49(3):1383-1396. doi: 10.1093/nar/gkab002.
9
Coordination of transcription, processing, and export of highly expressed RNAs by distinct biomolecular condensates.通过不同的生物分子凝聚体协调高度表达 RNA 的转录、加工和输出。
Emerg Top Life Sci. 2020 Dec 11;4(3):281-291. doi: 10.1042/ETLS20190160.
10
Phase Separation and Transcription Regulation: Are Super-Enhancers and Locus Control Regions Primary Sites of Transcription Complex Assembly?相分离和转录调控：超级增强子和基因座控制区是否为转录复合物组装的主要位点？
Bioessays. 2019 Jan;41(1):e1800164. doi: 10.1002/bies.201800164. Epub 2018 Nov 30.
Nucleic Acids Res. 1982 Dec 11;10(23):7809-18. doi: 10.1093/nar/10.23.7809.
4
The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.染色质中果蝇热休克基因的5'端对脱氧核糖核酸酶I高度敏感。
Nature. 1980 Aug 28;286(5776):854-60. doi: 10.1038/286854a0.
5
Unexpected relationships between four large deletions in the human beta-globin gene cluster.人类β-珠蛋白基因簇中四个大片段缺失之间的意外关系。
Cell. 1983 Dec;35(3 Pt 2):701-9. doi: 10.1016/0092-8674(83)90103-4.
6
Establishment of a near-tetraploid B-cell lymphoma line with duplication of the 8;14 translocation.建立具有8;14易位重复的近四倍体B细胞淋巴瘤细胞系。
Cancer Genet Cytogenet. 1984 May;12(1):39-50. doi: 10.1016/0165-4608(84)90006-2.
7
Erythroleukemia (K562) cells contain a functional beta-globin gene.红白血病（K562）细胞含有一个功能性β-珠蛋白基因。
Mol Cell Biol. 1984 Nov;4(11):2553-5. doi: 10.1128/mcb.4.11.2553-2555.1984.
8
Human globin gene expression in hybrid 2S MEL X human fibroblast cells.人珠蛋白基因在杂交的2S MEL X人成纤维细胞中的表达。
Somat Cell Mol Genet. 1984 Jul;10(4):399-407. doi: 10.1007/BF01535635.
9
Regulated expression of the human beta-globin gene family in murine erythroleukaemia cells.人β-珠蛋白基因家族在小鼠红白血病细胞中的调控表达。
Nature. 1983;305(5932):333-6. doi: 10.1038/305333a0.
10
The primary structure of the human epsilon-globin gene.人类ε-珠蛋白基因的一级结构。
Cell. 1980 Oct;21(3):621-6. doi: 10.1016/0092-8674(80)90425-0.