Huang Cheng, Shi Yan, Zhou Changming, Guo Lianying, Liu Guohui, Zhuang Yu, Li Guyue, Hu Guoliang, Liu Ping, Guo Xiaoquan
Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
School of Computer and Information Engineering, Jiangxi Agricultural University, Nanchang, China.
Front Microbiol. 2021 Sep 8;12:739577. doi: 10.3389/fmicb.2021.739577. eCollection 2021.
Copper (Cu) is an important trace element with a two-sided effect on the growth performance of animals, which depends on the timing and dosage of Cu addition, etc. The purpose of this study was to determine the effects of oral copper sulfate (CuSO, 350 ppm) on growth performance, cecal morphology, and its microflora of chickens ( = 60) after 30, 60, and 90 days. The results showed that after 90 days of copper exposure, the chickens lost weight, the cecum mucosa was detached, and vacuolation and inflammatory infiltration occurred at the base of the lamina propria. In addition, using the 16S rDNA sequencing method, we observed that copper exposure changed the richness and diversity of intestinal microorganisms. At the phylum level, and both significantly increased, while significantly decreased in the Cu group compared with control check (CK) group. At the genus level, the relative abundance of decreased significantly, while , and increased significantly after copper exposure, and the change in microflora was most significant at 90 days. Moreover, the relevance of genus-level bacteria was altered. PICRUST analysis revealed potential metabolic changes associated with copper exposure, such as infection and metabolic disorders of nutrients. To sum up, these data show that subchronic copper exposure not only affects the growth and development of chickens but also causes the imbalance of intestinal microflora, which may further induce metabolic disorders in chickens.
铜（Cu）是一种重要的微量元素，对动物的生长性能具有双向影响，这取决于铜添加的时机和剂量等因素。本研究的目的是确定口服硫酸铜（CuSO₄，350 ppm）对60只鸡在30天、60天和90天后的生长性能、盲肠形态及其微生物群落的影响。结果表明，铜暴露90天后，鸡体重减轻，盲肠黏膜脱落，固有层基部出现空泡化和炎性浸润。此外，使用16S rDNA测序方法，我们观察到铜暴露改变了肠道微生物的丰富度和多样性。在门水平上，与对照（CK）组相比，铜组的厚壁菌门（Firmicutes）和变形菌门（Proteobacteria）均显著增加，而拟杆菌门（Bacteroidetes）显著减少。在属水平上，铜暴露后，栖粪杆菌属（Faecalibacterium）的相对丰度显著降低，而肠杆菌属（Enterobacter）、志贺氏菌属（Shigella）和埃希氏菌属（Escherichia）显著增加，且微生物群落在90天时变化最为显著。此外，属水平细菌的相关性也发生了改变。PICRUST分析揭示了与铜暴露相关的潜在代谢变化，如感染和营养物质代谢紊乱。综上所述，这些数据表明，亚慢性铜暴露不仅影响鸡的生长发育，还会导致肠道微生物群落失衡，这可能进一步诱发鸡的代谢紊乱。
