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从朽木中分离得到的LBM 134及其潜在的纤维素分解能力。

LBM 134 isolated from rotten wood and its potential cellulolytic ability.

作者信息

Díaz Gabriela Verónica, Coniglio Romina Olga, Chungara Clara Inés, Zapata Pedro Darío, Villalba Laura Lidia, Fonseca María Isabel

机构信息

Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones "María Ebe Reca" CONICET. Facultad de Ciencias Exactas, Químicas y Naturales. Universidad Nacional de Misiones. Ruta, Posadas, Misiones, Argentina.

出版信息

Mycology. 2020 Sep 21;12(3):160-173. doi: 10.1080/21501203.2020.1823509. eCollection 2021.

Abstract

Aspergillus is a genus of filamentous and cosmopolitan fungi that includes important species for medical mycology, food, basic research and agro-industry areas. section are efficient producers of hydrolytic enzymes such as cellulases that are employed in the cellulose conversion. Hence, the search of new cellulolytic isolates and their correct identification is important for carrying out safe biotechnological processes. This study aimed to characterise the cellulolytic potential of sp. LBM 134, isolated from the Paranaense rainforest (Argentina) and to identify the isolate through a polyphasic approach. The fungus was identified as and its cellulolytic potential was evaluated by using Congo red technique and fluorescence plate assays for carboxymethyl cellulase, β-glucosidase and cellobiohydrolase, respectively. All three cellulase activities were positive; this bio-prospective positioned LBM 134 as a promising alternative for industries that require organisms capable of carrying out cellulosic biomass processing.

摘要

曲霉属是一类丝状且分布广泛的真菌,其中包含对医学真菌学、食品、基础研究和农业产业领域具有重要意义的物种。该属是纤维素酶等水解酶的高效生产者,这些酶用于纤维素转化。因此,寻找新的纤维素分解菌株并对其进行正确鉴定对于开展安全的生物技术过程至关重要。本研究旨在表征从阿根廷巴拉那雨林分离出的曲霉属LBM 134菌株的纤维素分解潜力,并通过多相方法鉴定该菌株。该真菌被鉴定为[具体名称未给出],并分别使用刚果红技术以及羧甲基纤维素酶、β-葡萄糖苷酶和纤维二糖水解酶的荧光平板测定法评估其纤维素分解潜力。所有三种纤维素酶活性均为阳性;这种生物勘探将LBM 134菌株定位为对需要能够进行纤维素生物质加工的生物体的行业有前景的替代选择。

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