Wuhan Mental Health Center, The Ninth Clinical School, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Department of Pathophysiology, School of Basic Medicine, Key Laboratory of Education Ministry of China for Neurological Disorders, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Front Cell Infect Microbiol. 2021 Sep 10;11:706961. doi: 10.3389/fcimb.2021.706961. eCollection 2021.
The poor oral health condition of individuals who abuse methamphetamine (MA) is well known. The roles of the oral and fecal microbiomes in addiction and nervous system diseases have been the focus of many studies. However, changes in the microbiota composition of MA users have not been reported. This was addressed in the present study in 20 MA users and 14 sex-matched healthy subjects. Saliva samples were collected and high-throughput 16S rRNA sequencing and bioinformatic analysis were performed to evaluate oral microbiome profiles. The results showed that species richness was significantly lower in the MA group than in the control group. Bacterial taxa that are known to be related to oral diseases such as Negativicutes, Veillonellaceae, Veillonella, and Selenomonadales had higher relative abundance in the MA group than in the control group, and the relative abundance of Prevotella melaninogenica-a putative etiologic agent of periodontal disease-was also higher. Avoiding MA use and improving oral hygiene practices over a short term (i.e., during hospitalization for 2 weeks) did not alter the oral microbiota composition of MA users. Although the causal relationship between changes in oral microbiome profile and MA abuse remains to be determined, our results suggest that oral disease prevention and treatment strategies are important for MA users.
滥用 methamphetamine（MA）者的口腔健康状况较差是众所周知的。口腔和粪便微生物组在成瘾和神经系统疾病中的作用一直是许多研究的焦点。然而，目前尚未有报道关于 MA 用户的微生物群落组成的变化。本研究纳入了 20 名 MA 用户和 14 名性别匹配的健康对照者，采集唾液样本并进行高通量 16S rRNA 测序和生物信息学分析，以评估口腔微生物组图谱。结果显示，MA 组的物种丰富度显著低于对照组。与口腔疾病相关的细菌分类群，如 Negativicutes、Veillonellaceae、Veillonella 和 Selenomonadales，在 MA 组中的相对丰度高于对照组，而牙周病潜在病原体 Prevotella melaninogenica 的相对丰度也较高。在短时间内（即住院治疗 2 周期间）避免 MA 使用和改善口腔卫生习惯并不能改变 MA 用户的口腔微生物群落组成。尽管口腔微生物组谱变化与 MA 滥用之间的因果关系仍有待确定，但我们的结果表明，口腔疾病预防和治疗策略对 MA 用户很重要。
