Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki 305-8686, Japan.
Center for Bioscience Research and Education, Utsunomiya University, Utsunomiya, Tochigi 321-8505, Japan.
G3 (Bethesda). 2021 Sep 27;11(10). doi: 10.1093/g3journal/jkab277.
Plant diseases caused by the Cercospora genus of ascomycete fungi are a major concern for commercial agricultural practices. Several Cercospora species can affect soybeans, such as Cercospora kikuchii which causes soybean leaf blight. Speciation in Cercospora on soybean has not been adequately studied. Some cryptic groups of Cercospora also cause diseases on soybean. Moreover, it has been known C. kikuchii population genetic structure is different between countries. Consequently, further genomic information could help to elucidate the covert differentiation of Cercospora diseases in soybean. Here, we report for the first time, a chromosome-level genome assembly for C. kikuchii. The genome assembly of 9 contigs was 34.44 Mb and the N50 was 4.19 Mb. Based on ab initio gene prediction, several candidates for pathogenicity-related genes, including 242 genes for putative effectors, 55 secondary metabolite gene clusters, and 399 carbohydrate-active enzyme genes were identified. The genome sequence and the features described in this study provide a solid foundation for comparative and evolutionary genomic analysis for Cercospora species that cause soybean diseases worldwide.
由子囊菌真菌尾孢属引起的植物病害是商业农业实践的主要关注点。一些尾孢菌物种会影响大豆，例如引起大豆叶斑病的尾孢菌。大豆上尾孢菌的种间分化尚未得到充分研究。一些隐群的尾孢菌也会引起大豆病害。此外，已知 C. kikuchii 在不同国家的种群遗传结构存在差异。因此，进一步的基因组信息可以帮助阐明大豆上尾孢菌病害的隐蔽分化。在这里，我们首次报道了 C. kikuchii 的染色体水平基因组组装。基因组组装由 9 个 contigs 组成，大小为 34.44 Mb，N50 为 4.19 Mb。基于从头预测基因，鉴定了几个与致病性相关的基因候选，包括 242 个假定效应物基因、55 个次生代谢物基因簇和 399 个碳水化合物活性酶基因。本研究中的基因组序列和特征为引起全球大豆病害的尾孢属物种的比较和进化基因组分析提供了坚实的基础。
