Väänänen P, Vaheri A
J Med Virol. 1979;3(4):245-52. doi: 10.1002/jmv.1890030402.
The hemolysis-in-gel (HIG) technique was adapted for rubella antibody determinations. Use of sucrose gradient purified virus and its coupling with CrCl3 to chicken erythrocytes resulted in gel plates that could be stored for several weeks and were suitable for reproducible antibody determinations. In a serological survey of young healthy adults the HIG values (range less than 2--13 mm) were in close correlation to those obtained by the HI test (less than 10 5o 320). The Hig test seems well suited for screening the need of vaccination. Seronegative sera (HIG less than 2,HI less than 10) gave without heat inactivation hemolysis zones ranging from 4 to 6.5 mm. Although the present rubella HIG test did not measure IgM antibodies, the test, by virtue of its accuracy and sensitivity--extending to antibody levels corresponding to HI titers 2--10--provides a simpler and more rapid means for diagnosis of rubella infections than the conventional HI and CF tests.
溶血凝胶（HIG）技术被应用于风疹抗体的测定。使用蔗糖梯度纯化病毒并将其与氯化铬偶联到鸡红细胞上，制成的凝胶板可保存数周，适用于重复性抗体测定。在一项针对年轻健康成年人的血清学调查中，HIG值（范围小于2 - 13毫米）与血凝抑制试验（HI试验）得到的值（小于10至320）密切相关。Hig试验似乎非常适合筛选疫苗接种需求。血清阴性血清（HIG小于2，HI小于10）在未经热灭活的情况下溶血区范围为4至6.5毫米。尽管目前的风疹HIG试验无法检测IgM抗体，但该试验凭借其准确性和敏感性——可检测到与HI滴度2 - 10相对应的抗体水平——为风疹感染的诊断提供了一种比传统HI试验和补体结合试验（CF试验）更简单、更快速的方法。
