School of Food and Biological Engineering, Jiangsu University, Zhenjiang, People's Republic of China.
School of Pharmacy, Jiangsu University, Zhenjiang, People's Republic of China.
Biomed Chromatogr. 2022 Jan;36(1):e5250. doi: 10.1002/bmc.5250. Epub 2021 Oct 17.
Dendrobium huoshanense is a Chinese medicinal herb that has high quality and excellent efficacy. However, the chemical basis of its activity is still unclear. Of note, Dendrobium officinale is the most widely utilized among the Dendrobium species. Therefore, the current study systematically investigated the chemical constituents of methanolic extracts and different polar fractions of aqueous extracts from the two herbs by HPLC-ESI-MS , and then compared in vitro antioxidant activities of their five different polar extracts. Consequently, 61 and 49 compounds were identified from D. huoshanense and D. officinale, respectively, of which 43 compounds were common to both species. In addition, 17 out of 22 different compounds were identified only in D. huoshanense. Moreover, the peak areas of some shared identical compounds of D. huoshanense were significantly larger than that of D. officinale. In vitro antioxidant evaluation results showed that the n-BuOH-soluble fraction of the two herbs exhibited remarkable antioxidant activities. Furthermore, the antioxidant activities of different fractions of D. huoshanense were separately superior to that of D. officinale, which may be attributed to its variable and high contents of flavonoids, bibenzyls and phenanthrenes. These results provide the evidence for the high quality and efficacy of D. huoshanense.
霍山石斛是一种具有高品质和卓越疗效的中药材。然而，其活性的化学基础仍不清楚。值得注意的是，铁皮石斛是石斛属植物中应用最广泛的一种。因此，本研究通过高效液相色谱-电喷雾质谱联用（HPLC-ESI-MS）系统地研究了这两种药材的甲醇提取物和水提物不同极性部位的化学成分，并比较了它们五种不同极性提取物的体外抗氧化活性。结果从霍山石斛和铁皮石斛中分别鉴定出 61 种和 49 种化合物，其中 43 种化合物为两种植物共有。此外，在霍山石斛中还鉴定出 22 种不同化合物中的 17 种。此外，一些共有相同化合物的峰面积霍山石斛显著大于铁皮石斛。体外抗氧化评价结果表明，两种药材的正丁醇可溶部分均具有显著的抗氧化活性。此外，霍山石斛不同部位的抗氧化活性均优于铁皮石斛，这可能归因于其黄酮类、联苄类和菲类化合物的种类多样且含量较高。这些结果为霍山石斛的高品质和高疗效提供了证据。
