Moreno J, Lipsky P E
J Clin Immunol. 1986 Jan;6(1):9-20. doi: 10.1007/BF00915359.
These studies were undertaken to examine the phenotype of the antigen-presenting cells (APC) circulating in human peripheral blood. Cells adherent to glass were found to be efficient APC, restoring antigen-induced 3H-thymidine incorporation to T4-positive T cells that had been rigorously depleted of contaminating APC. In order to identify the APC within the glass-adherent cells (AC), these cells were stained with a number of monocyte-specific monoclonal antibodies (Mo-Mab) including 3C10, 63D3, and 61D3, and the Mo-Mab-positive and -negative cells were separated with the fluorescence-activated cell sorter. This method of preparation yielded Mo-Mab(+) AC populations that were more than 98% positive for the relevant Mab when reanalyzed with the fluorescence-activated cell sorter. Less than 1% of the Mo-Mab(-) AC populations were positive when reanalyzed with the Mab used for the separation. However, each Mo-Mab(-) AC population was contaminated with variable numbers (4-60%) of Mo as detected by morphologic criteria, histochemical analysis for esterase activity, or staining with a different Mo-Mab. Both Mo-Mab(+) and (-) AC populations were found to be similarly effective APC, with as few as 500 cells/well supporting responses to streptokinase-streptodornase, tetanus toxoid, and Candida albicans antigen. In the absence of antigen, only 3C10(-), 63D3(-), or 61D3(-) AC consistently stimulated 3H-thymidine incorporation of autologous T4 cells; large numbers (greater than 5 X 10(3)/well) of APC were necessary to induce this response. These results support the conclusion that cells identified by Mo-specific Mab are capable of functioning as APC, inducing 3H-thymidine incorporation in response to exogenous antigens. However, Mo-Mab(+) AC are not unique in this activity since Mo-Mab(-) AC also appeared to be able to present antigen. These Mo-Mab(-) AC appear to contain the majority of cells inducing autologous T4-cell reactivity.
进行这些研究是为了检测循环于人类外周血中的抗原呈递细胞（APC）的表型。发现贴壁于玻璃的细胞是有效的APC，能使已彻底去除污染APC的T4阳性T细胞恢复抗原诱导的3H-胸腺嘧啶核苷掺入。为了鉴定贴壁于玻璃的细胞（AC）中的APC，这些细胞用多种单核细胞特异性单克隆抗体（Mo-Mab）染色，包括3C10、63D3和61D3，并用荧光激活细胞分选仪分离Mo-Mab阳性和阴性细胞。这种制备方法产生的Mo-Mab(+) AC群体在用荧光激活细胞分选仪重新分析时，相关单克隆抗体的阳性率超过98%。用用于分离的单克隆抗体重新分析时，不到1%的Mo-Mab(-) AC群体呈阳性。然而，通过形态学标准、酯酶活性的组织化学分析或用不同的Mo-Mab染色检测发现，每个Mo-Mab(-) AC群体都被数量不等（4% - 60%）的单核细胞污染。发现Mo-Mab(+)和(-) AC群体作为APC的效果相似，每孔低至500个细胞就能支持对链激酶-链道酶、破伤风类毒素和白色念珠菌抗原的反应。在无抗原情况下，只有3C10(-)、63D3(-)或61D3(-) AC能持续刺激自体T4细胞的3H-胸腺嘧啶核苷掺入；需要大量（大于5×10³/孔）的APC才能诱导这种反应。这些结果支持以下结论：由Mo特异性单克隆抗体鉴定的细胞能够作为APC发挥作用，对外源抗原诱导3H-胸腺嘧啶核苷掺入。然而，Mo-Mab(+) AC在这种活性方面并非唯一，因为Mo-Mab(-) AC似乎也能够呈递抗原。这些Mo-Mab(-) AC似乎包含诱导自体T4细胞反应性的大多数细胞。
