Department of Obstetrics and Gynecology, University Maternal Hospital, Cologne University, Cologne, Germany.
Department of Obstetrics and Gynecology, University Maternal Hospital, Cologne University, Cologne, Germany.
Cryobiology. 2021 Dec;103:1-6. doi: 10.1016/j.cryobiol.2021.09.009. Epub 2021 Sep 25.
Cryopreservation and re-transplantation of ovarian tissue after anticancer treatment is important medical technology. Today, during a pandemic, the risk of contamination of transplanted cells with SARS-CoV-2 virus is extremely high. Data about cryo-resistance (virulence and/or infectivity) of SARS-CoV-2 are limited. Analysis and systematization of literature data allow us to draw the following conclusions: 1) The cytoplasmic membrane of somatic cell, like envelope of corona viruses, consists of lipid bilayer and this membrane, like envelope of corona virus, contains membrane proteins. Thus, we can consider the cytoplasmic membrane of an ordinary somatic cell as a model of the envelope membrane of SARS-CoV-2. It is expected that the response of the virus to cryopreservation is similar to that of a somatic cell. SARS-CoV-2 is more poor-water and more protein-rich than somatic cell, and this virus is much more cryo-resistant. 2) The exposure of somatic cells at low positive temperatures increases a viability of these cells. The safety of the virus is also in direct proportion to the decrease in temperature: the positive effect of low temperatures on SARS-CoV-2 virus has been experimentally proven. 3) Resistance of SARS-CoV-2 to cryoprotectant-free cryopreservation is extremely high. The high viability rate of SARS-CoV-2 after freezing-drying confirms its high cryo-resistance. 4) The risk of SARS-CoV-2 infection after transplantation of cryopreserved ovarian tissues that have been contaminated with this virus, increases significantly. Our own experimental data on the increase in the viability of cancer cells after cryopreservation allow us to formulate a hypothesis about increasing of viability (virulence and/or infectivity) of SARS-CoV-2 virus after cryopreservation.
癌症治疗后进行卵巢组织的冷冻保存和再移植是一项重要的医学技术。如今，在大流行期间，移植细胞感染 SARS-CoV-2 病毒的风险极高。关于 SARS-CoV-2 的冷冻抗性（毒力和/或感染力）的数据有限。对文献数据的分析和系统化使我们得出以下结论：1）体细胞的细胞质膜，如同冠状病毒的包膜一样，由脂质双层组成，而该膜，如同冠状病毒的包膜一样，包含膜蛋白。因此，我们可以将普通体细胞的细胞质膜视为 SARS-CoV-2 的包膜膜的模型。预计病毒对冷冻保存的反应与体细胞相似。SARS-CoV-2 比体细胞更缺水且富含更多蛋白质，因此这种病毒更能抵抗冷冻。2）在较低的正温下暴露体细胞会增加这些细胞的活力。病毒的安全性也与温度的降低成正比：低温对 SARS-CoV-2 病毒的积极影响已在实验中得到证实。3）SARS-CoV-2 对无冷冻保护剂的冷冻保存具有极高的抗性。冷冻干燥后 SARS-CoV-2 的高存活率证实了其高冷冻抗性。4）卵巢组织在受到病毒污染的情况下进行冷冻保存并移植后感染 SARS-CoV-2 的风险显著增加。我们关于冷冻保存后癌细胞活力增加的实验数据使我们能够提出关于 SARS-CoV-2 病毒冷冻保存后活力（毒力和/或感染力）增加的假设。
