National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China.
The State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Sci Rep. 2017 Nov 2;7(1):14886. doi: 10.1038/s41598-017-14568-6.
The feed conversion ratio (FCR) is an essential economic trait for pig production, and is directly related to feed efficiency. Studies identifying the differential expression of functional genes involved in biological and molecular mechanisms in the intestine in relation to growth performance are rare. In this study, RNA-Seq was used to identify transcriptomes in caecal and colonic mucosal tissues in order to determine the differential expression of genes from two full-sibling pairs and two half-sibling pairs of Landrace finishing pigs with opposing FCR phenotypes. In total, 138 (comparison of high and low FCR in caecal mucosa), 64 (comparison of high and low FCR in colonic mucosa), and 165 (contrast between the caecal and colonic mucosa) differentially expressed genes were identified. Some of these genes were functionally related to energy and lipid metabolism, particularly short chain fatty acids metabolism, as well as gastrointestinal peristalsis and ion transport. Functional annotation were performed to identify differentially expressed genes, such as GUCA2A, GUCA2B, HSP70.2, NOS2, PCK1, SLCs, and CYPs, which may positively influence feed efficiency in Landrace pigs. These differentially expressed genes need to be further tested for candidate genes that are related to feed efficiency.
饲料转化率（FCR）是养猪生产的一个重要经济性状，与饲料效率直接相关。研究识别与生长性能相关的肠道中参与生物学和分子机制的功能基因的差异表达很少。在这项研究中，使用 RNA-Seq 来鉴定盲肠和结肠黏膜组织中的转录组，以确定具有相反 FCR 表型的两个全同胞对和两个半同胞对长白猪的盲肠和结肠黏膜组织中基因的差异表达。总共鉴定出 138 个（盲肠黏膜中高和低 FCR 的比较）、64 个（结肠黏膜中高和低 FCR 的比较）和 165 个（盲肠和结肠黏膜之间的对比）差异表达基因。其中一些基因与能量和脂质代谢，特别是短链脂肪酸代谢以及胃肠道蠕动和离子转运有关，功能相关。进行功能注释以识别差异表达基因，如 GUCA2A、GUCA2B、HSP70.2、NOS2、PCK1、SLCs 和 CYPs，它们可能对长白猪的饲料效率有积极影响。这些差异表达基因需要进一步测试与饲料效率相关的候选基因。
