Zhao Fangfang, Zhou Huitong, Li Shaobin, An Qingming, Fang Qian, Luo Yuzhu, Hickford Jon G H
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
International Science and Technology Cooperation Base of Meat Sheep and Meat Cattle Genetic Improvement in Northwest of China, Gansu Agricultural University, Lanzhou 730070, China.
Animals (Basel). 2021 Sep 14;11(9):2690. doi: 10.3390/ani11092690.
The glycogen synthase kinase 3 beta (GSK3β)-interacting protein (encoded by the gene ) is a small A-kinase anchoring protein, which complexes with GSK3βand protein kinase A (PKA) and acts synergistically with cAMP/PKA signaling to inhibit GSK3β activity. The protein plays a role in regulating glycogen metabolism, protein synthesis, the cell cycle, and in regulating gene expression. In this study, PCR-single strand conformation polymorphism (PCR-SSCP) analyses were used to screen for variation in exon 1 and exon 2 of in 840 New Zealand (NZ) Romney sheep. Two SSCP banding patterns representing two different nucleotide variants ( and ) were detected in an exon 1 region, whereas in an exon 2 region only one pattern was detected. Variants and of exon 1 had one non-synonymous nucleotide difference c.37A/G (p.Met13Val). The birthweight of sheep of genotype (5.9 ± 0.06 kg) was different ( = 0.023) to sheep of genotype (5.7 ± 0.06 kg) and (5.7 ± 0.06 kg). The hot carcass weight (HCW) of sheep of genotype (17.2 ± 0.22 kg) was different ( = 0.012) to sheep of genotype (17.6 ± 0.22 kg) and (18.0 ± 0.29 kg), and the fat depth at the 12th rib (V-GR) of sheep of genotype (7.7 ± 0.31 mm) was different ( = 0.016) to sheep of genotype (8.3 ± 0.30 mm) and (8.5 ± 0.39 mm). The results suggest that the c.37A/G substitution in ovine may affect sheep growth and carcass traits.
糖原合酶激酶3β（GSK3β）相互作用蛋白（由该基因编码）是一种小的A激酶锚定蛋白，它与GSK3β和蛋白激酶A（PKA）形成复合物，并与cAMP/PKA信号协同作用以抑制GSK3β活性。该蛋白在调节糖原代谢、蛋白质合成、细胞周期以及调节基因表达中发挥作用。在本研究中，采用聚合酶链反应-单链构象多态性（PCR-SSCP）分析方法，对840只新西兰罗姆尼绵羊的该基因外显子1和外显子2的变异进行筛选。在外显子1区域检测到代表两种不同核苷酸变异（和）的两种SSCP条带模式，而在外显子2区域仅检测到一种模式。外显子1的变异和有一个非同义核苷酸差异c.37A/G（p.Met13Val）。基因型绵羊的出生体重（5.9±0.06千克）与基因型绵羊（5.7±0.06千克）和（5.7±0.06千克）不同（P = 0.023）。基因型绵羊的热胴体重（HCW）（17.2±0.22千克）与基因型绵羊（17.6±0.22千克）和（18.0±0.29千克）不同（P = 0.012），基因型绵羊第12肋处的脂肪深度（V-GR）（7.7±0.31毫米）与基因型绵羊（8.3±0.30毫米）和（8.5±0.39毫米）不同（P = 0.016）。结果表明，绵羊该基因中的c.37A/G替换可能影响绵羊的生长和胴体性状。
