Zhang Jiaojiao, Wang Debao, Sun Jinyue, Sun Zhilan, Liu Fang, Du Lihui, Wang Daoying
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing 210014, China.
Foods. 2021 Sep 13;10(9):2171. doi: 10.3390/foods10092171.
This study evaluated the effect of the combination of ultrasound and phenyllactic acid (PLA) on inactivating and biofilm cells and determined the possible antibiofilm mechanism. and biofilm cells were separately treated with ultrasound (US, 270 W), phenyllactic acid (PLA, 0.5% and 1%), and their combination (US + 0.5% PLA, and US + 1% PLA) for 5, 10, 20, 30, and 60 min. Biofilm inactivation, polysaccharide, and respiratory chain dehydrogenase assays were conducted. US and PLA had a synergistic effect on inactivating bacterial cells in and biofilms. The combination of US and PLA significantly decreased the contents of soluble and insoluble polysaccharides and the activity of respiratory chain dehydrogenase in the biofilm cells compared to the single treatment. Confocal laser scanning microscopy, scanning electron microscopy, and intracellular adenosine-triphosphate (ATP) analyses indicated that the combination of US and PLA seriously destroyed the cell membrane integrity of the and biofilms and caused the leakage of intracellular ATP. These findings demonstrated the synergistic antibiofilm effect of US combined with PLA and offered a research basis for its application in the food industry.
本研究评估了超声与苯乳酸（PLA）联合使用对灭活细菌浮游细胞和生物膜细胞的效果，并确定了可能的抗生物膜机制。将细菌浮游细胞和生物膜细胞分别用超声（US，270 W）、苯乳酸（PLA，0.5%和1%）及其组合（US + 0.5% PLA和US + 1% PLA）处理5、10、20、30和60分钟。进行了生物膜灭活、多糖和呼吸链脱氢酶测定。超声和苯乳酸对灭活细菌浮游细胞和生物膜中的细菌细胞具有协同作用。与单一处理相比，超声和苯乳酸的组合显著降低了生物膜细胞中可溶性和不可溶性多糖的含量以及呼吸链脱氢酶的活性。共聚焦激光扫描显微镜、扫描电子显微镜和细胞内三磷酸腺苷（ATP）分析表明，超声和苯乳酸的组合严重破坏了细菌浮游细胞和生物膜的细胞膜完整性，并导致细胞内ATP泄漏。这些发现证明了超声与苯乳酸联合使用具有协同抗生物膜作用，并为其在食品工业中的应用提供了研究依据。
